Mono- to Multilayer Transition in Growing Bacterial Colonies.
Mono- to Multilayer Transition in Growing Bacterial Colonies.
复制标题
生长中的细菌菌落从单层到多层的转变。
DOI：
10.1103/physrevlett.123.178001
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发表时间：
2018
影响因子：
8.6
通讯作者：
L. Giomi
中科院分区：
文献类型：
--
作者：
Zhihong You;D. Pearce;A. Sengupta;L. Giomi
细菌菌落从单层结构向多层结构的转变是生物被膜发展的基本步骤。在不同的形态类型和物种中观察到，这种转变是在由数百个细胞组成的自由生长的细菌微克隆内触发的。利用数值模拟和分析模型相结合的方法，我们论证了这种转变源于生长诱导的面内活动应力和垂直恢复力之间的竞争，这是由于细胞-衬底相互作用造成的。利用一个简单的横向受限细胞链状群体，我们证明了当单个细胞对旋转变得不稳定时，转变集；因此，它是局域性的和机械确定性的。非同步细胞分裂使过程随机化，因此控制转变开始的所有关键参数都是连续分布的随机变量。在这里，我们证明了在大细胞数极限下，群体中第一次分裂的发生可以近似为泊松过程。这使我们能够近似计算与第一次挤压相关的位置和时间的概率分布函数。这样的泊松过程的速率可以被识别为相变的序参数，从而突出了其确定性和随机性的混合性质。
The transition from monolayers to multilayered structures in bacterial colonies is a fundamental step in biofilm development. Observed across different morphotypes and species, this transition is triggered within freely growing bacterial microcolonies comprising a few hundred cells. Using a combination of numerical simulations and analytical modeling, here we demonstrate that this transition originates from the competition between growth-induced in-plane active stresses and vertical restoring forces, due to the cell-substrate interactions. Using a simple chainlike colony of laterally confined cells, we show that the transition sets when individual cells become unstable to rotations; thus it is localized and mechanically deterministic. Asynchronous cell division renders the process stochastic, so that all the critical parameters that control the onset of the transition are continuously distributed random variables. Here we demonstrate that the occurrence of the first division in the colony can be approximated as a Poisson process in the limit of large cell numbers. This allows us to approximately calculate the probability distribution function of the position and time associated with the first extrusion. The rate of such a Poisson process can be identified as the order parameter of the transition, thus highlighting its mixed deterministic-stochastic nature.
影响因子：
19.6
作者：
Beroz, Farzan;Yan, Jing;Wingreen, Ned S.
通讯作者：
Wingreen, Ned S.