Manipulating Pyruvate Decarboxylase by Addition of Enzyme Regulators during Fermentation of Rhizopus oryzae to Enhance Lactic Acid Production
Manipulating Pyruvate Decarboxylase by Addition of Enzyme Regulators during Fermentation of Rhizopus oryzae to Enhance Lactic Acid Production
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DOI：
10.1007/s12010-014-1155-0
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发表时间：
2014-08
影响因子：
3
通讯作者：
S. Thitiprasert;P. Songserm;Wasinee Boonkong;S. Sooksai;K. Kodama;N. Thongchul
中科院分区：
文献类型：
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作者：
S. Thitiprasert;P. Songserm;Wasinee Boonkong;S. Sooksai;K. Kodama;N. Thongchul
Ethanol was found as the major by-product in lactate fermentation byRhizopus oryzae. Several methods have been conducted in order to limit ethanol formation, thus increasing the lactate yield. The direct way to suppress ethanol production can be done by inhibition of the responsible enzymes in the related pathway. Pyruvate decarboxylase (PDC) and alcohol dehydrogenase (ADH) are responsible for ethanol production inR. oryzae. Shunting the ethanol production pathway by targeting at PDC was attempted in this study. Three compounds including 4-methylpyrazole, glyoxylic acid, and 3-hydroxypyruvate with the in vitro reversible inhibitory effect on PDC were selected from the literature and were used to regulate the living cell ofR. oryzaeduring the fermentation. The results show that 0.1 mM 4-methylpyrazole of which the structure resembled a thiazolium ring in thiamine diphosphate, PDC cofactor, and 1.0 μm 3-hydroxypyruvate, pyruvate analog, effectively hampered ethanol production. Further observation on the enzyme expression indicated that these two regulators not only targeted PDC but also caused changes in ADH and lactate dehydrogenase (LDH) activities. This was perhaps due to the living cell ofR. oryzaethat responded to the presence of the regulators to balance the pyruvate flux and subsequently maintain its metabolic activities.
