Construction of SSR-based chromosome map in bunching onion (Allium fistulosum)
Construction of SSR-based chromosome map in bunching onion (Allium fistulosum)
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DOI：
10.1007/s00122-008-0849-5
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发表时间：
2008-09
影响因子：
5.4
通讯作者：
H. Tsukazaki;K. Yamashita;S. Yaguchi;S. Masuzaki;H. Fukuoka;J. Yonemaru;H. Kanamori;I. Kono;T. Hằng;M. Shigyo;A. Kojima;T. Wako
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作者：
H. Tsukazaki;K. Yamashita;S. Yaguchi;S. Masuzaki;H. Fukuoka;J. Yonemaru;H. Kanamori;I. Kono;T. Hằng;M. Shigyo;A. Kojima;T. Wako
我们利用225株的F_2群体构建了丛生洋葱(Allium fiumulosumL.，2n=416)的连锁图谱。该图谱由17个连锁群组成，包含212个簇状洋葱SSR标记和42个鳞茎洋葱(A.Cepal.)SSR、Indel、CAPS或dCAPS标记，覆盖2,069 cm。这是首次报道以SSR标记为主要标记的葱属连锁图谱。利用103个锚定标记[81个簇状洋葱SSR，11个鳞茎洋葱SSR和11个非鳞茎洋葱SSR(1个Indel，9个CAPS和1个dCAPS)]，对它们的染色体进行了鉴定。洋葱/欧拉。利用几种不同的大蒜附加系，在17个连锁群中有16个连结到8条基本染色体上。CEPA。
We have constructed a linkage map of bunching onion (Allium fistulosumL., 2n= 16) using an F2population of 225 plants. The map consists of 17 linkage groups with 212 bunching onion SSR markers and 42 bulb onion (A. cepaL.) SSR, InDel, CAPS or dCAPS markers, covering 2,069 cM. This is the first report of a linkage map mainly based on SSR markers in the genusAllium. With the 103 anchor markers [81 bunching onion SSRs, 11 bulb onion SSRs and 11 bulb onion non-SSRs (1 InDel, 9 CAPSs and 1 dCAPS)] whose chromosome assignments were identified inA. cepaand/orA. fistulosum, via the use of several kinds ofAlliumalien addition lines, 16 of the 17 linkage groups were connected to the 8 basic chromosomes ofA. cepa.