Survey of fresh vegetables for nematodes, amoebae, and Salmonella.

Survey of fresh vegetables for nematodes, amoebae, and Salmonella.
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新鲜蔬菜线虫、变形虫和沙门氏菌调查。

DOI:
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发表时间:
1984
期刊:
Journal - Association of Official Analytical Chemists
影响因子:
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通讯作者:
N. G. Risty
N. G. Risty
中科院分区:
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文献类型:
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作者:
R. A. Rude;G. J. Jackson;J. W. Bier;T. Sawyer;N. G. Risty

文献摘要

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通过对从批发和零售来源获得的沙拉蔬菜进行为期2年的调查,估计了线虫、变形虫和沙门氏菌属细菌的污染。检查的蔬菜有黄瓜、卷心菜、莴苣、芹菜、胡萝卜、萝卜、西红柿、蘑菇、花椰菜和菠菜。通过Nacconol-ether离心法回收线虫卵和幼虫。部分虫卵经鉴定为寄生性蛔虫;大多数线虫幼虫被认为是土壤中的物种。通过用蒸馏水冲洗蔬菜,去除冲洗水,并将沉淀物转移到先前生长有细菌菌苔的琼脂平板上,回收了阿米巴;滋养体被鉴定为潜在的致病物种--多食阿米巴原虫,A. rhysodes和A.卡氏阿米巴是盘子里最常见的阿米巴沙门氏菌从50个样品中的4个中生长。
Contamination by nematodes, amoebae, and bacteria of the genus Salmonella was estimated in a 2-year survey of salad vegetables obtained from wholesale and retail sources. The vegetables examined were cucumbers, cabbage, lettuce, celery, carrots, radishes, tomatoes, mushrooms, cauliflower, and spinach. Nematode eggs and larvae were recovered by the Nacconol-ether centrifugation method. Some nematode eggs were identified as parasitic Ascaris sp.; the majority of larval nematodes were thought to be soil-dwelling species. Amoebae were recovered by rinsing the vegetables with distilled water, centrifuging the rinse water, and transferring the sediment to agar plates on which a bacterial lawn had previously been grown; trophozoites identified as the potentially pathogenic species--Acanthamoeba polyphaga, A. rhysodes, and A. castellanii--were the most common amoebae recovered on the plates. Salmonella spp. were grown from 4 of 50 samples.