Burn wounds infected with Pseudomonas aeruginosa triggers weight loss in rats.
Burn wounds infected with Pseudomonas aeruginosa triggers weight loss in rats.
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DOI：
10.1186/1471-2482-5-19
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发表时间：
2005-09-17
期刊：
影响因子：
1.9
通讯作者：
Wang, Stewart C
中科院分区：
文献类型：
--
作者：
Steinstraesser, Lars;Burkhard, Olaf;Wang, Stewart C
背景：尽管烧伤的治疗有了显著的改善，但感染仍然是烧伤患者的一个严重风险。本研究的目的是阐明急性烧伤感染或不感染对细胞因子profiles.METHODS的影响：Sprague-Dawley大鼠（n = 21）随机分为三组：1）烧伤，2）烧伤和感染或3）假烧伤。监测体重并收集血液用于细胞因子ELISA、LPS定量和外周血分析。动物被处死后6或12 days.RESULTS：感染的动物表现出显着的体重减轻，直到第6天烧伤后相比，单独烧伤。烧伤后48小时内，感染烧伤组早期内毒素和TNF-α水平升高。与此相反，抗炎细胞因子IL-10的显著上调发生在临床病程后期，并与从体重loss.CONCLUSION恢复相关：我们的研究结果表明，在感染的存在下，你得到一个全身炎症反应可能是由于短暂的内毒素血症，只看到在感染组。相比之下，烧伤和感染都有晚期IL-10（汽车）反应，然后与感染组恢复正常体重相关。
BACKGROUND: Despite dramatic improvements in the management of burns, infection still remains a serious risk for the burn patient. The aim of this study was to shed light on the impact of acute burn injury with or without infection on cytokine profiles.METHODS: Sprague-Dawley rats (n = 21) were randomized into three groups: 1) burn only 2) burn and infection or 3) sham burn. Weight was monitored and blood was collected for cytokine ELISA, LPS quantification, and peripheral blood analysis. Animals were sacrificed either after 6 or 12 days.RESULTS: Infected animals showed substantial weight loss until day 6 post-burn as compared to burn alone. Endotoxin and TNF-alpha levels were elevated early in the infected burn group within 48 hours post-burn. In contrast, significant up-regulation of the anti-inflammatory cytokine IL-10 occurred later in the clinical course and was associated with the recovery from weight loss.CONCLUSION: Our results suggest that in the presence of infection, you get a SIRS response possibly due to transient endotoxemia that is only seen in the infection group. In contrast, both burn and infection get a late IL-10 (CARS) response, which is then associated with a return to normal weight in the infection group.