Subcellular localization of hematoporphyrin derivative in bladder tumor cells in culture.
Subcellular localization of hematoporphyrin derivative in bladder tumor cells in culture.
复制标题
培养的膀胱肿瘤细胞中血卟啉衍生物的亚细胞定位。
DOI：
10.1111/j.1751-1097.1990.tb01736.x
复制
发表时间：
1990
影响因子：
3.3
通讯作者：
Lin,CW
中科院分区：
文献类型：
--
作者：
Shulok,JR;Wade,MH;Lin,CW
Mitochondria have been implicated as a primary subcellular site of porphyrin localization and photodestruction. However, other organelles including the cell membrane, lysosomes and nucleus have been shown to be damaged by hematoporphyrin derivative (HpD) photosensitized destruction as well. In this study we attempted to follow the translocation of the fluorescent components of HpD in human bladder tumor cells (MGH‐U1) in culture to determine whether specific subcellular localization occurs over time. Following a 30 min exposure to HpD the cellular fluorescence was examined immediately and 1, 2, 4, and 24 h after HpD removal using fluorescence microscopy and an interactive laser cytometer. Thein vitrotranslocation of dye appeared to be fairly rapid with fluorescence present at the cell membrane and later (1‐2 h) within a perinuclear area of the cytoplasm. To determine whether HpD had become concentrated into a specific subcellular organelle, these fluorescence distribution patterns were compared with fluorescent marker dyes specific for mitochondria, endoplasmic reticulum and other membranous organelles. The HpD fluorescence did not appear to be as discrete as the dyes specific for mitochondria or endoplasmic reticulum but appeared similar to the diffuse cytomembrane stain. Finally, the interaction between the fluorescent components of HpD and the cellular constituents was evaluated using a “fluorescence redistribution after photobleaching” technique. The results indicated that the mean lateral diffusion for HpD in MGH‐U1 cells was 1.05 times 10−8cm2/s, a rate closer to that of lipid diffusion (10K) than that of protein diffusion (10−10). Under our experimental conditions, these studies indicated that upon entering the cell the fluorescent components of HpD rapidly translocated to lipophilic cytomembranous structures but did not appear to partition into a specific subcellular site.
登录
查看更多内容
影响因子：
8.8
作者：
Gantchev, TG;Brasseur, N;vanLier, JE
通讯作者：
vanLier, JE
光动力疗法降低癌症结肠细胞粘附性和转移潜力
DOI：
--
发表时间：
1995
期刊：
Research in experimental medicine. Zeitschrift fur die gesamte experimentelle Medizin einschliesslich experimenteller Chirurgie
影响因子：
--
作者：
V. Vonarx;M. Foultier;L. Xavier de Brito;L. Anasagasti;L. Morlet;T. Patrice
通讯作者：
T. Patrice
光生物学中的时间分辨荧光。
DOI：
10.1016/1011-1344(88)85033-4
发表时间：
1988
期刊：
Journal of photochemistry and photobiology. B, Biology
影响因子：
--
作者：
H. Schneckenburger;H. Seidlitz;J. Eberz
通讯作者：
J. Eberz
原卟啉 IX 的光敏作用可抑制癌细胞与基质的附着。
DOI：
--
发表时间：
2004
期刊：
Biochemical and Biophysical Research Communications - BBRC
影响因子：
--
作者：
A. Uzdensky;A. Juzeniene;E. Kolpakova;G. Hjortland;P. Juzenas;J. Moan
通讯作者：
J. Moan
一种简单的体外方法，用于检测单线态氧并使用碱性磷酸酶比较光动力活性。
影响因子：
1.4
作者：
H. S. Yadav;V. Jain
通讯作者：
V. Jain