Expression of vimentin and glial fibrillary acidic protein in ethylnitrosourea-induced rat gliomas and glioma cell lines
Expression of vimentin and glial fibrillary acidic protein in ethylnitrosourea-induced rat gliomas and glioma cell lines
复制标题
乙基亚硝基脲诱导的大鼠胶质瘤和胶质瘤细胞系中波形蛋白和胶质纤维酸性蛋白的表达
作者：
G. Reifenberger;T. Bilzer;R. Seitz;W. Wechsler
摘要采用免疫组织化学方法研究了移植应用乙基亚硝基脲（ENU）诱导的104例CDF大鼠胶质瘤中胶质原纤维酸性蛋白（GFAP）和vimentin的表达。在许多星形细胞肿瘤细胞中，尤其是在许多肿瘤中形成间变性髓母细胞瘤样灶的小胶质瘤细胞中，波形蛋白的免疫反应性是突出的。在少突胶质瘤中，除了一些大的多形性少突胶质瘤中混杂着波形蛋白阳性的胶质瘤细胞外，大多数肿瘤细胞为波形蛋白阴性。GFAP免疫反应性仅在一小部分肿瘤星形胶质细胞中检测到，在少数例外情况下，一些少突胶质肿瘤细胞染色呈阳性。神经丝和细胞角蛋白抗体免疫组化显示enu诱导的胶质瘤细胞未染色，而所有少突胶质瘤细胞HNK-1阳性。来自enu诱导胶质瘤的两个大鼠胶质瘤细胞克隆RG2和F98的免疫细胞学和免疫印迹研究显示，在单层培养和同基因脑内移植肿瘤中，vimentin的表达显著。F98还显示出GFAP阳性细胞的一部分，特别是在融合培养和脑肿瘤中。另一方面，RG2在培养中几乎没有表现出GFAP免疫反应性，但在脑内肿瘤中显示出单个GFAP阳性肿瘤细胞。我们的结果揭示了enu诱导的大鼠胶质瘤和两种广泛使用的胶质瘤细胞系的细胞分化的更精确的图像。他们强调了实验大鼠胶质瘤的异质性，胶质瘤可能包括向少突胶质或星形胶质表型分化的不同阶段的细胞。
SummaryThe expression of glial fibrillary acidic protein (GFAP) and vimentin was investigated immuno-histochemically in 104 experimental gliomas induced by transplancental application of ethylnitrosourea (ENU) in CDF rats. Immunoreactivity for vimentin was prominent in many astrocytic tumor cells and especially in small glioma cells forming anaplastic medulloblastoma-like foci in many tumors. The majority of tumor cells in oligodendroglial tumors were vimentin negative, except for some of the large polymorphous oligodendrogliomas which contained intermingled vimentin positive glioma cells. GFAP immunoreactivity was detectable only in a low fraction of tumor astrocytes and in a few exceptional cases some oligodendroglial tumor cells stained positive. Immunohistochemistry with antibodies against neurofilaments and cytokeratins revealed no staining in tumor cells of ENU-induced gliomas, while all oligoden-drogliomatous tumors stained positive for HNK-1. Immunocytological and immunoblot investigations of the two rat glioma cell clones RG2 and F98, which are both derived from ENU-induced gliomas, showed a prominent expression of vimentin in monolayer cultures and in syngeneic intracerebral transplantation tumors. F98 additionally demonstrated a fraction of GFAP positive cells especially in confluent cultures and in intracerebral tumors. RG2, on the other hand, exhibited virtually no GFAP immunoreactivity in culture but showed individual GFAP positive tumor cells in intracerebral tumors. Our results revealed a more precise picture of the cellular differentiation in ENU-induced rat gliomas and in two widely used glioma cell lines. They underline the heterogeneity of experimental rat gliomas which may comprise cells at different stages of differentiation towards the oligodendroglial or astroglial phenotype.
免疫过氧化物酶组织化学在人类神经肿瘤学中的最新应用。
影响因子：
4.6
作者：
Perentes,E;Rubinstein,LJ
通讯作者：
Rubinstein,LJ