RFLP facilitated analysis of tiller and leaf angles in rice (Oryza sativa L.)
RFLP facilitated analysis of tiller and leaf angles in rice (Oryza sativa L.)
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DOI：
10.1023/a:1003533001014
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发表时间：
1999-01-01
期刊：
影响因子：
1.9
通讯作者：
Stansel, JW
中科院分区：
文献类型：
--
作者：
Li, ZK;Paterson, AH;Stansel, JW
株型是与水稻等谷类作物产量潜力相关的重要复合性状。利用115个分布良好的RFLP标记构建了一张完整的水稻分蘖角、叶片角和剑叶角的遗传连锁图，并对2418个水稻品种（系）的F-2和F-4后代进行了分析。莴苣n.粳稻'Lemont'和spp.籼稻特青。Lemont/特青F-2群体3个株型性状的变异主要由1对主效基因（Ta）和11个QTL控制。主效基因Ta位于第9染色体上RZ 228和RG 667之间，对分蘖角的表型变异贡献率为47.5%，对叶角和剑叶角都有较大的多效性效应。该基因加4个QTL可解释分蘖角基因型变异的69.1%。另外还发现了8个控制叶角和剑叶角的QTL，分别解释了52.0%和66.4%的基因型变异。Ta和3个QTL（QFla 2、QFla 5和QFla 7）对相关株型性状的影响存在明显差异，表明它们可能在水稻植株的不同发育阶段有差异表达，或者可能是影响这些性状的不同基因的聚类。在分子标记辅助选择程序中，可以通过有意操纵这些QTL来改良水稻的株型，从而提高水稻的产量。
Plant type is an important composite trait associated with the yield potential in rice and other cereal crops. Several characters associated with the plant type of modern rice cultivars including tiller angle, leaf and flag leaf angle, were investigated using a complete linkage map with 115 well distributed RFLP markers and progeny testing of 2418 F-2 derived F-4 lines from a cross between O. sativa ssp. japonica cv. 'Lemont' and spp. indica cv. 'Teqing'. One major gene (Ta) and 11 QTLs were largely responsible for the tremendous variation of the three plant type characters in the Lemont/Teqing F-2 population. The major gene, Ta, located between RZ228 and RG667 on chromosome 9, accounted for 47.5% of the phenotypic variation in tiller angle and had large pleiotropic effects on both leaf and flag leaf angles. This gene plus four QTLs accounted for 69.1% of the genotypic variation in tiller angle. Eight additional QTLs for leaf and flag leaf angles were also identified, which collectively explained 52.0 and 66.4% of the genotypic variation of these traits. Ta and three QTLs (QFla2, QFla5 and QFla7) apparently affected the related plant type characters differently, suggesting their possible differential expression in different developmental stages of rice plants or possibly clustering of different genes affecting these traits. Plant type, and consequently grain yield of rice, may be improved by deliberately manipulating these QTLs in a marker-assisted selection program.