Morphological, genetic, chemical and ecophysiological characterisation of two Microcystis aeruginosa isolates from the Vistula Lagoon, southern Baltic
Morphological, genetic, chemical and ecophysiological characterisation of two Microcystis aeruginosa isolates from the Vistula Lagoon, southern Baltic
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DOI：
10.5697/oc.52-1.127
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发表时间：
2010-01-01
期刊：
影响因子：
2.9
通讯作者：
Plinski, Marcin
中科院分区：
文献类型：
--
作者：
Mazur-Marzec, Hanna;Browarczyk-Matusiak, Grazyna;Plinski, Marcin
维斯瓦潟湖（波罗的海南部）是一个浅而高度富营养化的水体，经常有以微囊藻和水藻为主的蓝藻大量繁殖。本研究分离了两株微囊藻菌株MK10.10和MAKR0205。分离株的形态在细胞大小和形成聚集体的能力方面有显著差异。根据16S rRNA序列和16S- 23s内部转录间隔物（ITS）序列，将两株菌株归类为铜绿微囊藻。然而，只有一株菌株MK10.10具有负责微囊藻毒素生物合成的活性基因，并且只有该菌株产生微囊藻毒素。光照、温度和盐度等环境因素对毒素产生的影响较小。在实验所采用的培养条件下，毒株MK10.10的生物量始终较低。采用四极杆飞行时间液相色谱/串联质谱（QTOF-LC/MS/MS）对MK10.10产生的微囊藻毒素（MC）变异体进行结构分析。基于分子离子谱和碎片谱，鉴定出三种毒素分别为MC-LR、MC-VR和MC-HIIR。我们的研究证实了一些形态标准可以用于初步评估微囊藻华的潜在毒性。
The Vistula Lagoon (southern Baltic Sea) is a shallow and highly eutrophic water body, with frequent blooms of cyanobacteria dominated by Microcystis and Anabaena species. Two Microcystis strains, MK10.10 and MAKR0205, isolated from the lagoon were characterised in this work. The morphology of the isolates differed significantly with respect to cell size and their ability to form aggregates. Based on the 16S rRNA sequence and 16S-23S internal transcribed spacer (ITS) sequence, both isolates were classified as Microcystis aeruginosa. However, only one isolate, MK10.10, possessed the racy genes responsible for microcystin biosynthesis and only this strain produced microcystins. The effects of environmental factors, such as light, temperature and salinity, on toxin production turned out to be minor. Under the culture conditions used in the experiments, the biomass of the toxic MK10.10 was always lower. Hybrid quadrupole-time-of-flight liquid chromatography/tandem mass spectrometry (QTOF-LC/MS/MS) was used to elucidate the structure of the microcystin (MC) variants produced by MK10.10. Based on molecular ion and fragmentation spectra, the toxins were identified as MC-LR, MC-VR, and MC-HIIR. Our study confirmed that some morphological criteria could be useful in preliminarily assessing the potential toxicity of a Microcystis bloom.