Characterization of sialosylated Lewisx as a new tumor-associated antigen.
Characterization of sialosylated Lewisx as a new tumor-associated antigen.
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发表时间：
1984-11
期刊：
影响因子：
11.2
通讯作者：
K. Fukushima;M. Hirota;P. Terasaki;A. Wakisaka;H. Togashi;D. Chia;N. Suyama;Y. Fukushi;E. Nudelman;S. Hakomori
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文献类型：
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作者：
K. Fukushima;M. Hirota;P. Terasaki;A. Wakisaka;H. Togashi;D. Chia;N. Suyama;Y. Fukushi;E. Nudelman;S. Hakomori
已经制备了与唾液酸Lex反应但不与唾液酸莱亚反应的单克隆抗体CSLEX 1。CSLEX1抗原具有与Lex相似的组织分布，特征性地出现在肾近端小管和粒细胞上。它与肿瘤相关，因为测试的34个肿瘤系中的14个（41%）与CSLEX1抗体反应，并且测试的74个肿瘤组织中的50个（68%）与抗体反应。神经氨酸酶处理后组织的免疫过氧化物酶染色丧失表明抗体与唾液酸衍生物反应。该抗体在固相放射免疫分析中与唾液酸乳糖岩藻五糖（III）神经酰胺和唾液酸二岩藻糖神经节苷脂（6B）反应。这些结果表明CSLEX1表位具有以下结构：（分子式：见正文）该结构以前不知道与肿瘤相关。
A monoclonal antibody CSLEX1 which reacts with sialosyl Lex but not with sialosyl Lea has been produced. The CSLEX1 antigen has a tissue distribution similar to that of Lex, appearing characteristically in the proximal tubules of the kidney and on granulocytes. It is tumor associated in that 14 of 34 (41%) of tumor lines tested reacted with the CSLEX1 antibody, and 50 of 74 (68%) of tumor tissues tested reacted with the antibody. Loss of immunoperoxidase staining of tissues after neuraminidase treatment showed that the antibody is reacting to sialyl derivatives. The antibody reacted in solid-phase radioimmunoassay to sialosyllactofucopentaosyl(III)ceramide and sialosyldifucosylganglioside (6B). These results indicate that the CSLEX1 epitope has the following structure: (formula: see text) This structure had not previously been known to be tumor associated.