Enhanced immunogenicity of gp120 protein when combined with recombinant DNA priming to generate antibodies that neutralize the JR-FL primary isolate of human immunodeficiency virus type 1
Enhanced immunogenicity of gp120 protein when combined with recombinant DNA priming to generate antibodies that neutralize the JR-FL primary isolate of human immunodeficiency virus type 1
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DOI：
10.1128/jvi.79.12.7933-7937.2005
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发表时间：
2005-06-01
影响因子：
5.4
通讯作者：
Lu, S
中科院分区：
文献类型：
--
作者：
Wang, SX;Arthos, J;Lu, S
人类免疫缺陷病毒1型疫苗的开发需要策略，以提高针对病毒主要分离株的中和抗体应答。在这里，我们研究了重组DNA引发，然后亚基蛋白加强作为一种策略，以产生中和抗体。基于质粒的和重组蛋白包膜（Env）糖蛋白免疫原均来源于原代病毒分离株，用基因枪接种gp 120或gp 140 DNA疫苗，然后用重组gp 120蛋白加强免疫的兔血清能够中和JR-FL。单独的DNA疫苗和单独的gp 120蛋白都不能产生可检测的抗该病毒的中和抗体应答。使用gp 120 DNA和gp 140 DNA进行初免的中和抗体应答相似。结果表明，Env DNA引发，然后gp 120蛋白加强提供了一个优势，无论是单独的方法，产生可检测的中和抗体反应，对不容易中和的主要分离株。
Strategies are needed for human immunodeficiency virus type 1 vaccine development that improves the neutralizing antibody response against primary isolates of the virus. Here we examined recombinant DNA priming followed by subunit protein boosting as a strategy to generate neutralizing antibodies. Both plasmid-based and recombinant protein envelope (Env) glycoprotein immunogens were derived from a primary viral isolate, JR-FL. Serum from rabbits immunized with either gp120 or gp140 DNA vaccines delivered by gene gun inoculation followed by recombinant gp120 protein boosting was capable of neutralizing JR-FL. Neither the DNA vaccines alone nor the gp120 protein alone generated a detectable neutralizing antibody response against this virus. Neutralizing antibody responses using gp120 DNA and gp140 DNA for priming were similar. The results suggest that Env DNA priming followed by gp120 protein boosting provides an advantage over either approach alone for generating a detectable neutralizing antibody response against primary isolates that are not easily neutralized.