Biochemical and morphological characterization of SEPT1 in mouse brain
Biochemical and morphological characterization of SEPT1 in mouse brain
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DOI：
10.1007/s00795-020-00248-4
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发表时间：
2020-03-07
影响因子：
1.8
通讯作者：
Nagata, Koh-ichi
中科院分区：
文献类型：
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作者：
Ito, Hidenori;Morishita, Rika;Nagata, Koh-ichi
Septins是一个高度保守的GTP酶家族，在从酵母到人类的多种生物体中鉴定。在哺乳动物中，神经组织大量含有septins，并且已经报道了septins与神经系统疾病如阿尔茨海默病和帕金森病的关联。然而，septins在大脑发育中的作用尚未完全了解。在这项研究中，我们产生了针对小鼠SEPT 1的特异性抗体，并进行了SEPT 1的生化和形态学特征。当通过蛋白质印迹分析小鼠脑发育期间SEPT 1的表达谱时，我们发现SEPT 1的表达在出生后开始增加，并且增加持续到出生后第22天。小鼠脑的亚细胞分级分离和随后的蛋白质印迹分析揭示了SEPT 1在突触组分中的分布。免疫荧光分析显示SEPT 1定位于原代培养的小鼠海马神经元的突触。免疫组织化学方法也发现了SEPT 1在小鼠脑内突触的分布。这些结果表明，SEPT 1参与了各种突触事件，如信号传导，神经递质释放和突触形成/维持。
Septins are a highly conserved family of GTPases which are identified in diverse organisms ranging from yeast to humans. In mammals, nervous tissues abundantly contain septins and associations of septins with neurological disorders such as Alzheimer's disease and Parkinson's disease have been reported. However, roles of septins in the brain development have not been fully understood. In this study, we produced a specific antibody against mouse SEPT1 and carried out biochemical and morphological characterization of SEPT1. When the expression profile of SEPT1 during mouse brain development was analyzed by western blotting, we found that SEPT1 expression began to increase after birth and the increase continued until postnatal day 22. Subcellular fractionation of mouse brain and subsequent western blot analysis revealed the distribution of SEPT1 in synaptic fractions. Immunofluorescent analyses showed the localization of SEPT1 at synapses in primary cultured mouse hippocampal neurons. We also found the distribution of SEPT1 at synapses in mouse brain by immunohistochemistry. These results suggest that SEPT1 participates in various synaptic events such as the signaling, the neurotransmitter release, and the synapse formation/maintenance.