Single-cell transcriptomics in planaria: new tools allow new insights into cellular and evolutionary features.
Single-cell transcriptomics in planaria: new tools allow new insights into cellular and evolutionary features.
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DOI：
10.1042/bst20210825
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发表时间：
2022-10-31
影响因子：
3.9
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中科院分区：
文献类型：
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单细胞转录组学彻底改变了生物学，允许量化单个细胞中的基因表达。由于每个单细胞都含有细胞类型特异性mRNA，因此这些技术能够对细胞身份进行分类。因此，单细胞方法已被用于探索不同生物体（包括淡水真涡虫）的细胞类型（单细胞图谱）。现今，真涡虫是单细胞生物学中最重要的动物模型之一。它们已经在单细胞水平上使用大多数可用的单细胞方法学方法研究了十多年。这些方法包括基于板的方法，例如qPCR、纳米滴方法和原位条形码化方法。由于这些研究，我们现在对真涡虫细胞类型及其分化轨迹有了很好的了解。Planarian的再生特性和其他特征，如它们的发育可塑性和无性繁殖能力，确保了Planarian单细胞生物学的另一个十年尚未到来。在这里，我们回顾了这些特征，单细胞转录组学获得的新的生物学见解，并概述了未来的前景。
Single-cell transcriptomics has revolutionised biology allowing the quantification of gene expression in individual cells. Since each single cell contains cell type specific mRNAs, these techniques enable the classification of cell identities. Therefore, single cell methods have been used to explore the repertoire of cell types (the single cell atlas) of different organisms, including freshwater planarians. Nowadays, planarians are one of the most prominent animal models in single cell biology. They have been studied at the single cell level for over a decade using most of the available single cell methodological approaches. These include plate-based methods, such as qPCR, nanodroplet methods and in situ barcoding methods. Because of these studies, we now have a very good picture of planarian cell types and their differentiation trajectories. Planarian regenerative properties and other characteristics, such as their developmental plasticity and their capacity to reproduce asexually, ensure that another decade of single cell biology in planarians is yet to come. Here, we review these characteristics, the new biological insights that have been obtained by single-cell transcriptomics and outline the perspectives for the future.