Porous Lactose-Modified Chitosan Scaffold for Liver Tissue Engineering: Influence of G alactose Moieties on Cell Attachment and Mechanical Stability
Porous Lactose-Modified Chitosan Scaffold for Liver Tissue Engineering: Influence of G alactose Moieties on Cell Attachment and Mechanical Stability
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用于肝组织工程的多孔乳糖修饰壳聚糖支架：半乳糖部分对细胞附着和机械稳定性的影响
DOI：
10.1155/2016/2862738
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Qi Chang Zheng
中科院分区：
文献类型：
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作者：
Birong Wang;Qinggang Hu;Tao Wan;Fengxiao Wang;Le Cui;Shaobo Hu;Bo Gong;Min Li;Qi Chang Zheng
半乳糖基化壳聚糖（CTS）作为肝组织工程支架已得到广泛应用。然而，半乳糖部分取代度（DS）对细胞附着和机械稳定性的影响尚不清楚。本研究利用希夫碱反应和NaBH4的还原作用合成了半乳糖不同DS的乳糖修饰壳聚糖（Lact - CTS），并用FTIR对其进行了表征。通过电位滴定法，Lact‐CTS‐1、Lact‐CTS‐2和Lact‐CTS‐3的DS分别为19.66%、48.62%和66.21%。由于半乳糖配体受体的识别，肝细胞在CTS和Lact - CTS膜上的附着随着CTS链上半乳糖部分的增加而增强；然而，Lact - CTS - 3的机械稳定性降低，导致了过度的亲水性，这一点通过固滴法得到了证明。然后，采用冷冻干燥技术制备三维乳酸- CTS支架。扫描电镜结果表明，支架的孔隙结构均匀，具有良好的连通性，孔径以100μm居多；然而，溶血实验表明，Lact - CTS - 3支架提取物溶液明显破坏红细胞，表明Lact - CTS - 3的DS过高降低了支架的机械稳定性，增加了支架的毒性。综上所述，半乳糖含量为48.62%的Lact‐CTS‐2能够促进细胞附着，具有良好的生物相容性和机械稳定性，是一种很有前景的肝组织工程材料。
Galactosylated chitosan (CTS) has been widely applied in liver tissue engineering as scaffold. However, the influence of degree of substitution (DS) of galactose moieties on cell attachment and mechanical stability is not clear. In this study, we synthesized the lactose‐modified chitosan (Lact‐CTS) with various DS of galactose moieties by Schiff base reaction and reducing action of NaBH4, characterized by FTIR. The DS of Lact‐CTS‐1, Lact‐CTS‐2, and Lact‐CTS‐3 was 19.66%, 48.62%, and 66.21% through the method of potentiometric titration. The cell attachment of hepatocytes on the CTS and Lact‐CTS films was enhanced accompanied with the increase of galactose moieties on CTS chain because of the galactose ligand‐receptor recognition; however, the mechanical stability of Lact‐CTS‐3 was reduced contributing to the extravagant hydrophilicity, which was proved using the sessile drop method. Then, the three‐dimensional Lact‐CTS scaffolds were fabricated by freezing‐drying technique. The SEM images revealed the homogeneous pore bearing the favorable connectivity and the pore sizes of scaffolds with majority of 100μm; however, the extract solution of Lact‐CTS‐3 scaffold significantly damaged red blood cells by hemolysis assay, indicating that exorbitant DS of Lact‐CTS‐3 decreased the mechanical stability and increased the toxicity. To sum up, the Lact‐CTS‐2 with 48.62% of galactose moieties could facilitate the cell attachment and possess great biocompatibility and mechanical stability, indicating that Lact‐CTS‐2 was a promising material for liver tissue engineering.