Isolation and Culture of Primary Human CNS Neural Cells
Isolation and Culture of Primary Human CNS Neural Cells
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DOI：
10.1007/978-1-60761-292-6_5
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发表时间：
2010-01-01
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影响因子：
--
通讯作者：
Antel, Jack P.
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文献类型：
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作者：
Blain, Manon;Miron, Veronique E.;Antel, Jack P.
我们目前的方法，分离和培养细胞从成人和胎儿的人中枢神经系统。所使用的细胞分离程序也非常适合于获得用于立即基于RNA和蛋白质的分析的样品，特别是随着使所需材料量最小化的技术变得越来越可用。通常用于啮齿动物材料的其他组织培养技术可适用于人成人和胎儿组织，例如海马切片培养和神经球测定（Dorr等J Neuroimmunol 167：204-209，2005; Chojnacki等Ann Neurol 64：127-142，2008）。
We present our current methods for isolating and culturing cells from the adult and fetal human CNS. The cell isolation procedures used are also well suited to obtain samples for immediate RNA- and protein-based analyses, particularly as techniques to minimize the amount of material needed become increasingly available. Further tissue culture techniques Commonly used for rodent material can be adapted to human adult and fetal tissues such as the hippocampal slice culture and the neurosphere assay (Dorr et al. J Neuroimmunol 167:204-209, 2005; Chojnacki et al. Ann Neurol 64:127-142, 2008).