In Vivo Assessment of Peripheral Nerve Regeneration by Diffusion Tensor Imaging
In Vivo Assessment of Peripheral Nerve Regeneration by Diffusion Tensor Imaging
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DOI：
10.1002/jmri.22442
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发表时间：
2011-03-01
影响因子：
4.4
通讯作者：
Kubo, Toshikazu
中科院分区：
文献类型：
--
作者：
Morisaki, Shinsuke;Kawai, Yuko;Kubo, Toshikazu
目的：评估弥散张量成像（DTI）在体内评估周围神经再生的敏感性。我们评估了神经损伤后 DTI 参数的变化和组织学分析，以检查大鼠坐骨神经的变性和再生情况。 材料和方法：对于磁共振成像（MRI），将 16 只大鼠随机分为两组：P 组（永久挤压；n = 7）和 T 组（暂时挤压；it = 9）。术前对右腿进行连续 MRI 检查，然后在挤压伤后 1、2、3 和 4 周的时间点进行检查。各向异性分数（FA）、轴向扩散率（λ（平行于））和径向扩散率（λ（垂直于））的变化被量化。对于组织学分析，对轴突数量和有髓轴突面积进行量化。结果：在退变期观察到 FA 减少和 lambda（垂直）增加，在再生期观察到 FA 增加和 lambda（垂直）减少。 FA和λ(垂直)的变化与组织学变化密切相关，包括轴突和髓磷脂再生。结论：DTI参数，特别是λ(垂直)可以作为周围神经再生的良好指标，可作为多种神经系统疾病的无创诊断工具。
Purpose: To evaluate the sensitivity of diffusion tensor imaging (DTI) in assessing peripheral nerve regeneration in vivo. We assessed the changes in the DTI parameters and histological analyses after nerve injury to examine degeneration and regeneration in the rat sciatic nerves.Materials and Methods: For magnetic resonance imaging (MRI), 16 rats were randomly divided into two groups: group P (permanently crushed; n = 7) and group T (temporally crushed; it = 9). Serial MRI of the right leg was performed before the operation, and then performed at the timepoints of 1, 2, 3, and 4 weeks after the crush injury. The changes in fractional anisotropy (FA), axial diffusivity (lambda(parallel to)), and radial diffusivity (lambda(perpendicular to)) were quantified. For histological analyses, the number of axons and the myelinated axon areas were quantified.Results: Decreased FA and increased lambda(perpendicular to) were observed in the degenerative phase, and increased FA and decreased lambda(perpendicular to) were observed in the regenerative phase. The changes in FA and lambda(perpendicular to) were strongly correlated with histological changes, including axonal and myelin regeneration.Conclusion: DTI parameters, especially lambda(perpendicular to), can be good indicators for peripheral nerve regeneration and can be applied as noninvasive diagnostic tools for a variety of neurological diseases.