Mammalian homologues of the Drosophila Son of sevenless gene map to murine chromosomes 17 and 12 and to human chromosomes 2 and 14, respectively.
Mammalian homologues of the Drosophila Son of sevenless gene map to murine chromosomes 17 and 12 and to human chromosomes 2 and 14, respectively.
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果蝇 Son of Sevenless 基因的哺乳动物同源物分别映射到小鼠 17 号和 12 号染色体以及人类 2 号和 14 号染色体。
DOI：
10.1006/geno.1993.1421
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发表时间：
1993
期刊：
影响因子：
4.4
通讯作者：
Bowtell,DD
中科院分区：
文献类型：
--
作者：
Webb,GC;Jenkins,NA;Largaespada,DA;Copeland,NG;Fernandez,CS;Bowtell,DD
治疗基因的激活突变通常存在于广泛的人类肿瘤中。我们最近克隆了两个哺乳动物基因，Son of sevenless 1 （mSos1）和Son of sevenless 2 (mSos2)，它们的蛋白产物似乎是ras蛋白的重要正调控因子。考虑到Sos蛋白在ras调控中的作用，以及肿瘤细胞中频繁出现的活化的drasall等位基因，我们有兴趣确定这些osgenes是否也可能在肿瘤细胞中的DNA重排中被不适当地激活。为了研究这种可能性，我们利用遗传连锁分析和染色体传播的原位杂交相结合，确定了小鼠和人类sos1和sos2基因的染色体位置。我们发现小鼠的esos1和sos2基因分别定位于染色体17E和12C3.3-D，人类的对应基因分别定位于染色体2p21-2p2和14q21。人类和小鼠的osloci位点都不接近已知突变或肿瘤细胞中显示一致核型异常的区域。
Activating mutations in therasgenes are commonly found in a wide range of human tumors. We recently cloned two mammalian genes,Son of sevenless 1 (mSos1)andSon of sevenless 2 (mSos2), whose protein products appear to be important positive regulators of ras proteins. Given the proposed role of Sos proteins in ras regulation, and the frequent occurrence of activatedrasalleles in tumor cells, we were interested in determining whether theSosgenes may also be activated inappropriately by DNA rearrangement in tumor cells. To investigate this possibility, we have determined the chromosomal locations of both the mouse and the humanSos1andSos2genes, using a combination of genetic linkage analysis andin situhybridization to chromosomal spreads. We find that the murineSos1andSos2genes map to chromosomes 17E and 12C3.3-D and their human counterparts to chromosomes 2p21-2p2 and 14q21, respectively. Neither the human nor the mouseSosloci map close to known mutations or to regions showing consistent karyotypic abnormalities in tumor cells.