Three-Dimensional Protein Cage Array Capable of Active Enzyme Capture and Artificial Chaperone Activity
Three-Dimensional Protein Cage Array Capable of Active Enzyme Capture and Artificial Chaperone Activity
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DOI：
10.1021/acs.nanolett.9b01148
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发表时间：
2019-06-01
期刊：
影响因子：
10.8
通讯作者：
Heddle, Jonathan G.
中科院分区：
文献类型：
--
作者：
Chakraborti, Soumyananda;Korpi, Antti;Heddle, Jonathan G.
开发用于包封活性酶货物的蛋白质笼及其随后排列成可控的三维阵列是非常期望的。然而，货物捕获通常具有挑战性，因为在温和条件下难以实现蛋白质笼的可逆组装/拆卸。在这里，我们表明，通过使用一种不寻常的铁蛋白笼蛋白，在温和的条件下进行可重复的组装，我们可以实现可逆的填充与蛋白质货物，包括活性酶。我们证明，这些填充笼可以排列在三维晶格中，并有一个额外的伴侣样的效果，增加热稳定性和酶活性的封装酶。
Development of protein cages for encapsulation of active enzyme cargoes and their subsequent arrangement into a controllable three-dimensional array is highly desirable. However, cargo capture is typically challenging because of difficulties in achieving reversible assembly/disassembly of protein cages in mild conditions. Herein we show that by using an unusual ferritin cage protein that undergoes triggerable assembly under mild conditions, we can achieve reversible filling with protein cargoes including an active enzyme. We demonstrate that these filled cages can be arrayed in three-dimensional crystal lattices and have an additional chaperone-like effect, increasing both thermostability and enzymatic activity of the encapsulated enzyme.