CHITINASE OF SERRATIA MARCESCENS
CHITINASE OF SERRATIA MARCESCENS
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DOI：
10.1139/m69-122
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发表时间：
1969-01-01
影响因子：
2.8
通讯作者：
REESE, ET
中科院分区：
文献类型：
--
作者：
MONREAL, J;REESE, ET
发现粘质沙雷氏菌是 100 种测试产生几丁质酶的生物体中最活跃的。液化肠杆菌产生的酶几乎同样多。在最佳条件下，4-6 天内即可获得高产量的几丁质酶。 S.marcescensenzyme 系统位于细胞外，由内切几丁质酶、壳二糖酶和“结晶”几丁质水解所需的因子 (CH1) 组成。因此，它与绿色木霉的纤维素酶系统非常相似，并且不同于仅作用于降解形式的几丁质的几丁质分解系统。粘质链霉菌滤液中不存在其他水解酶，这使得该系统成为确定几丁质酶作为溶解剂的作用的有用系统。
Serratia marcescenswas found to be the most active organism of 100 tested for the production of chitinase.Enterobacter liquefaciensproduced nearly as much enzyme. Under optimal conditions high yields of chitinase were obtained in 4–6 days. TheS.marcescensenzyme system is extracellular and is composed of an endochitinase, a chitobiase, and a factor (CH1) required for the hydrolysis of "crystalline" chitin. As such, it closely resembles the cellulase system ofTrichoderma viride, and differs from those chitinolytic systems which act only on degraded forms of chitin. The absence of other hydrolytic enzymes in theS.marcescensfiltrates makes this a useful system for determining the role of chitinase as a lytic agent.