[Variable number of tandem repeats as an evidence of engraftment status after allogeneic peripheral blood stem cell transplantation].
[Variable number of tandem repeats as an evidence of engraftment status after allogeneic peripheral blood stem cell transplantation].
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发表时间：
2000-11
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通讯作者：
J. Wang;J. Wang;W. Zhang
中科院分区：
文献类型：
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作者：
J. Wang;J. Wang;W. Zhang
目的根据红细胞类型、染色体核型和可变串联重复序列数（VNTR）评价异基因外周血干细胞移植（allo-PBSCT）后患者的植入状态。方法采用聚合酶链反应（PCR）技术，对15例外周血造血干细胞移植（PBSCT）后患者的VNTR基因3个位点进行体外扩增，检测其多态性。同时对7例性别不匹配患者进行了染色体核型分析，对3例ABO血型不匹配患者进行了红细胞抗原转换分析。结果15例患者VNTR分析显示11例完全嵌合体，4例混合嵌合体。7例性别不匹配的患者，染色体核型分析显示其供体类型。至于血型分析，两个月后，第一个病人变成了他的捐赠者的血型。1例患者在移植后7个月表现为混合状态。结论PCR扩增VNTR位点检测异基因外周血干细胞移植后移植物的植入状态，方法简便、灵敏。这项技术特别适用于接受过同性别、同血型、同HLA型干细胞移植的患者。用几个不同的VNTR基因座进行检查将提高辨别力。
OBJECTIVE To evaluate engraftment status of patients after allogeneic peripheral blood stem cell transplantation (allo-PBSCT) based on red blood cell type, karyotype and variable number of tandem repeats (VNTR). METHODS Amplification of 3 loci of VNTR in vitro by polymerase chain reaction (PCR) was preformed to detect the polymorphism of 15 patients post-PBSCT. Furthermore, analysis of karyotype in 7 sex-mismatched patients and red blood cell antigen switching in 3 ABO blood type mismatched patients were also employed. RESULTS VNTR analysis in 15 patients revealed complete chimerism in 11 patients and mixed chimerism in 4. In the 7 sex-mismatched patients, karyotype analysis showed types of their donors. As for blood type analysis, it was after 2 months that the first patient transformed to his donor's type. One patient showed mixed status 7 months after transplantation. CONCLUSION Amplification of VNTR loci by PCR for documenting engraftment status after allo-PBSCT is simple and sensitive. This technology is especially suitable for patients who have received stem cells with the same sex, same blood type and identical HLA type. Examination with several different VNTR loci will improve the power of discrimination.