A Complex Composed of Tup1 and Ssn6 Represses Transcription in Vitro*
A Complex Composed of Tup1 and Ssn6 Represses Transcription in Vitro*
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DOI：
10.1074/jbc.272.17.11193
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发表时间：
1997-04
期刊：
影响因子：
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通讯作者：
M. Redd;M. Arnaud;A. Johnson
中科院分区：
文献类型：
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作者：
M. Redd;M. Arnaud;A. Johnson
酿酒酵母Tup1蛋白是WD重复序列家族中的一员，包含参与转录抑制的蛋白质。Tup1与SSn6蛋白一起抑制酵母中的多种基因，包括细胞类型特异性基因和葡萄糖抑制基因。Tup1和SSn6通过与序列特异的DNA结合蛋白相互作用而被招募到这些特定的基因集中。在这项工作中，提纯了含有SSN6和Tup1的蛋白质复合体，以确定其组成。该复合体的大小估计为440 kDa。Tup1和SSn6都是磷酸蛋白质，是该复合体中仅有的化学计量比含量的蛋白质。我们还证明了这个纯化的复合体在体外实验中抑制转录。
The Saccharomyces cerevisiae Tup1 protein is a member of a family of WD repeat containing proteins that are involved in repression of transcription. Tup1, along with the Ssn6 protein, represses a wide variety of genes in yeast including cell type-specific and glucose-repressed genes. Tup1 and Ssn6 are recruited to these specific gene sets by interaction with sequence-specific DNA binding proteins. In this work, a protein complex containing Ssn6 and Tup1 was purified to determine its composition. The size of the complex is estimated to be 440 kDa. Tup1 and Ssn6, which are both phosphoproteins, are the only proteins present in stoichiometric amounts in the complex. We also demonstrate that this purified complex represses transcription in an in vitro assay.