PEGylated magnetic Prussian blue nanoparticles asa multifunctional therapeutic agent for combined targeted photothermal ablation and pH-triggered chemotherapy of tumour cells.
PEGylated magnetic Prussian blue nanoparticles asa multifunctional therapeutic agent for combined targeted photothermal ablation and pH-triggered chemotherapy of tumour cells.
复制标题
DOI：
10.1016/j.jcis.2017.09.027
复制
发表时间：
2018
影响因子：
9.9
通讯作者：
P. Xue;Lihong Sun;Qian Li;Lei Zhang;Zhigang Xu;Chang Ming Li;Yuejun Kang
中科院分区：
文献类型：
--
作者：
P. Xue;Lihong Sun;Qian Li;Lei Zhang;Zhigang Xu;Chang Ming Li;Yuejun Kang
多功能纳米制剂已成为流行的和有价值的药物，用于有效的癌症治疗。此外，开发具有优异的生物相容性、高效性、特异性靶向和组合治疗效果的治疗剂的趋势越来越大。在这项研究中，我们提出了一种简单的技术来合成聚乙二醇化（聚乙二醇修饰）磁性普鲁士蓝（PB）纳米粒子与封装阿霉素（DOX），简称为Fe3O4@PB/PEG/DOX纳米粒子，结合靶向光热消融和pH触发化疗的肿瘤细胞。通过薄膜水合过程实现Fe3O4@PB核壳结构的PEG化;通过疏水相互作用将DOX负载到纳米胶囊中。一项体外研究表明，在酸性条件下增加药物释放，模拟温和的酸性肿瘤微环境。此外，纳米复合材料表现出超顺磁性，有助于改善局部磁场引导的治疗效果。细胞毒性研究表明，突出的光热化疗组合对HeLa细胞的影响，归因于靶向光热效应介导的pH触发的细胞摄取的DOX。具体而言，在用纳米剂（DOX = 10 μg mL-1）和近红外线照射处理后，HeLa细胞的存活率降低至8.5%，表明明显的体外肿瘤抑制效果。这项研究提出了一种有效和靶向癌症治疗的纳米平台，这可能导致开发用于癌症治疗的多功能纳米药物载体。
Multifunctional nanoagents have become popular and valuable pharmaceuticals for effective cancer treatment. Moreover, there is an increasing tendency to develop therapeutic agents with excellent biocompatibility, high efficiency, specific targeting and combinatorial treatment effects. In this study, we proposed a facile technique to synthesize PEGylated (polyethylene glycol modified) magnetic Prussian blue (PB) nanoparticles with encapsulated doxorubicin (DOX), abbreviated as Fe3O4@PB/PEG/DOX NPs, for combined targeted photothermal ablation and pH-triggered chemotherapy of tumour cells. The PEGylation of Fe3O4@PB core-shell structure was achieved through a thin-film hydration process; DOX was loaded into the nanocapsule via hydrophobic interactions. Anin vitrostudy indicated increased drug release under acidic conditions, mimicking mild acidic tumour microenvironments. Additionally, the nanocomposites exhibited superparamagnetism, contributing to an improved therapeutic effect guided by a localized magnetic field. Cytotoxicity studies demonstrated outstanding photothermal-chemotherapy combinatorial effects on HeLa cells, attributed to the targeted photothermic effect mediated by the pH-triggered cellular uptake of DOX. Specifically, the viability of HeLa cells decreased to 8.5% after treatment with the nanoagent (DOX = 10 μg mL−1) and near infrared irradiation, indicating an evident tumour inhibition effectin vitro. This study presented a nanoplatform for efficient and targeted cancer treatment, which may lead to the development of multifunctional nanodrug vehicles for cancer therapy.