Movement of ions and small solutes across endothelium and epithelium of perfused rabbit lungs.
Movement of ions and small solutes across endothelium and epithelium of perfused rabbit lungs.
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离子和小溶质穿过灌注兔肺的内皮和上皮的运动。
DOI：
10.1152/jappl.1986.60.1.100
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Hukkanen,J
中科院分区：
文献类型：
--
作者：
Effros,RM;Mason,GR;Silverman,P;Reid,E;Hukkanen,J
使用原位和分离的充满液体的兔肺来研究空气空间和血管室之间的指示物的传输。将这些指示剂放置在灌注液或空气空间中，并在 1 小时灌注期间定期从灌注液中收集样本。一小时结束时，将流体从空气空间室泵入串联管中，并测定空气空间和灌注液中的指示剂浓度。将指示剂引入空气空间后一小时，溶质浓度的相对下降为（从最大下降到最小下降排列）：[14C]尿素大于36Cl-=125I-大于22Na+大于[3H]甘露醇。灌注液中指示剂出现的相对速率相似。当将指示剂置于灌注液中时，在空气空间浓度的增加中观察到类似的关系，但灌注液中 22Na+ 的损失与 36Cl- 和 125I- 的损失相似。灌注液中所有指示剂的损失是空气空间中损失的两倍或更多，并且虽然约30分钟内灌注液中[3H]甘露醇的损失与22Na+的损失相似，但随后的损失要慢得多。很少有 125I-白蛋白穿过组织屏障，空气空间中 125I-白蛋白浓度的微小变化表明几乎没有发生液体运动。这些研究表明，上皮细胞对溶质的渗透性低于内皮细胞，并且允许阴离子以比 22Na+ 更快的速度通过。（摘要截断为 250 字）
In situ and isolated fluid-filled rabbit lungs were used to study the transport of indicators between the air space and vascular compartments. These indicators were placed in either the perfusate or air spaces and samples were collected from the perfusate at intervals during a 1-h perfusion period. At the end of the hour, fluid was pumped out of the air space compartment into serial tubes and indicator concentrations were determined in both the air space and perfusion fluids. One hour after introducing the indicators into the air space, the relative decreases in solute concentration were (arranged from the greatest to the least decline): [14C]urea greater than 36Cl- = 125I- greater than 22Na+ greater than [3H]mannitol. The relative rates at which the indicators appeared in the perfusate were similar. When the indicators were placed in the perfusate, a similar relationship was observed in the increase in air space concentrations, but the loss of 22Na+ from the perfusate was similar to those of 36Cl- and 125I-. Losses of all indicators from the perfusate were two or more times those from the air spaces, and although the loss of [3H]mannitol from the perfusate was similar to that of 22Na+ for about 30 min, subsequent loss was much slower. Very little 125I-albumin traversed the tissue barrier, and the small changes in the concentrations of 125I-albumin in the air spaces suggested that little fluid movement had occurred. These studies suggest that the epithelium is less permeable to solutes than the endothelium and permits passage of anions at a faster rate than 22Na+.(ABSTRACT TRUNCATED AT 250 WORDS)