Mode of action of a non-pathogenic Fusarium oxysporum strain against Verticillium dahliae using Real Time QPCR analysis and biomarker transformation
Mode of action of a non-pathogenic Fusarium oxysporum strain against Verticillium dahliae using Real Time QPCR analysis and biomarker transformation
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DOI：
10.1016/j.biocontrol.2009.01.010
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发表时间：
2009-07-01
影响因子：
4.2
通讯作者：
Paplomatals, Epaminondas J.
中科院分区：
文献类型：
--
作者：
Pantelides, Iakovos S.;Tjamos, Sotirios E.;Paplomatals, Epaminondas J.
黄萎病是一种危害广泛的草本和木本植物寄主的毁灭性病害。它是由土传真菌大丽轮枝菌引起的。管理战略主要侧重于预防措施。在以前的研究中，一个非致病性尖孢镰刀菌菌株，指定为F2，从抑制堆肥修正案中分离的功效，已被报道，以减少在温室和田间条件下茄子黄萎病症状的发展;此外，寄生或寄生被排除使用双重培养测试。在本研究中，我们研究了F2对大丽轮枝菌的作用方式。为此目的，分别用EGFP和DsRed 2报告基因转化F2和大丽轮枝菌菌株，以便可视化它们在茄子根表面上的存在。此外，通过真实的时间QPCR分析监测两种真菌进入植物维管系统的衍生物。结果表明，F2代除大丽轮枝菌外，其余菌株均沿着细胞间连接处在根表定殖。同时，QPCR分析表明，施用F2降低了大丽轮枝菌血管定殖水平，沿着疾病严重程度。在分根实验中，证明了F2不触发茄子对大丽轮枝菌的防御机制。因此，F2对棉花黄萎病菌的作用机制可能主要是对根表空间或养分的竞争。(C)2009 Elsevier Inc. All rights reserved.
Verticillium wilt is a devastating disease of a wide range of herbaceous and woody plant hosts. It is incited by the soilborne fungus Verticillium dahliae. Management strategies are mainly focused on preventive measures. In a previous study, the efficacy of a non-pathogenic Fusarium oxysporum strain, designated as F2, isolated from a suppressive compost amendment, has been reported to reduce Verticillium wilt symptom development in eggplants under greenhouse and field conditions; in addition, antibiosis or parasitism were ruled out by using a dual culture test. In the present study, we investigated the mode of action of F2 against V. dahliae. For this purpose, the F2 and V. dahliae strains were transformed with the EGFP and DsRed2 reporter genes respectively, so as to visualize their presence on the root surface of eggplants. In addition, the ramification of both fungi into the plant vascular system was monitored by Real Time QPCR analysis. It was shown that F2 colonizes the root surface along the intercellular junctions excluding V. dahliae from the same ecological niche. In parallel, QPCR analysis showed that application of F2 reduces the levels of V. dahliae vascular colonization along with the disease severity. In a split root experiment it was demonstrated that F2 does not trigger the defense mechanisms of eggplants against V. dahliae. Therefore, it seems that competition for space or nutrients on the root surface are the main mechanism of action of F2 against V. dahliae. (C) 2009 Elsevier Inc. All rights reserved.