Amine uptake and metabolism by endothelium of pig pulmonary artery and aorta.
Amine uptake and metabolism by endothelium of pig pulmonary artery and aorta.
复制标题
猪肺动脉和主动脉内皮对胺的吸收和代谢。
作者：
A. Junod;C. Ody
测定猪肺动脉、主动脉内皮细胞及肺切片对5-羟色胺（5HT）和l-去甲肾上腺素（NE）的摄取和代谢。氧化脱胺和饱和摄取机制的证据，对寒冷、丙咪嗪和无NA+介质敏感，在肺切片、肺和主动脉内皮中获得5HT，通过微孔过滤器吸收或暴露于胶原酶从管腔分离。然而，对于NE，仅在肺切片中可以证明类似的摄取。在主动脉和肺内皮中，NE被代谢，但其摄取不受丙咪嗪、无Na+培养基和高底物浓度的影响。因此，主动脉和肺内皮具有相似的特性，并表现出5HT，但不表现出NE，一种类似于肺毛细血管的摄取。
The uptake and metabolism of 5-hydroxytryptamine (5HT) and l-norepinephrine (NE) were measured in endothelial cells of pig pulmonary artery and aorta, as well as in lung slices. Evidence for oxidative deamination and for a saturable uptake mechanism, sensitive to cold, imipramine, and NA+-free medium was obtained for 5HT in lung slices and in the pulmonary as well as in the aortic endothelium, separated from the lumen by absorption to Millipore filters or by exposure to collagenase. For NE, however, a similar type of uptake could be deomonstrated in lung slices only. In the aortic and pulmonary endothelium, NE was metabolized, but its uptake was not influenced by imipramine, Na+-free medium, and high substrate concentration. The aortic and pulmonary endothelium have, therefore, similar properties and exhibit for 5HT, but not for NE, a type of uptake similar to that existing in lung capillaries.