Centrally administered relaxin-3 induces Fos expression in the osmosensitive areas in rat brain and facilitates water intake
Centrally administered relaxin-3 induces Fos expression in the osmosensitive areas in rat brain and facilitates water intake
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DOI：
10.1016/j.peptides.2010.02.020
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发表时间：
2010-06-01
期刊：
影响因子：
3
通讯作者：
Ueta, Yoichi
中科院分区：
文献类型：
--
作者：
Otsubo, Hiroki;Onaka, Tatsushi;Ueta, Yoichi
松弛蛋白-3基因是利用人类基因组数据库检测到的胰岛素超家族的新成员，在大脑和睾丸中大量存在。脑室注射松弛素-3可刺激食物摄取。脑室注射松弛素(与人类的松弛素-2相同)会影响加压素的分泌和饮酒行为。松弛蛋白-3部分结合松弛蛋白家族多肽受体1，后者是松弛蛋白的特异性受体。因此，我们假设松弛素-3在体液平衡中有生理作用。然而，松弛素-3在体液平衡中的作用仍不清楚。在本研究中，我们揭示了侧脑室注射松弛素-3在大鼠下丘脑和脑室周围器官，包括终板血管器、正中视前核、视上核(SON)、穹隆下器(SFO)和室旁核(PVN)，都有密集的Fos样免疫反应(Fos-LI)，这与中枢调节体液平衡有关。侧脑室注射松弛素-3(54、180和540pmoL/只)也可诱导SON、SFO和PVN c-fos基因表达显著增加，且呈剂量依赖关系。此外，侧脑室注射松弛素-3(180pmol/只)可显著增加大鼠的摄水量，其作用与松弛素-2(180pmol/只)相当。这些结果提示，侧脑室注射松弛素-3可激活脑内渗透敏感区，在调节体液平衡中起重要作用。(C)2010 Elsevier Inc.保留所有权利。
The expression of the relaxin-3 gene, detected as a new member of the insulin superfamily using human genomic databases, is abundantly present in the brain and testis. Intracerebroventricularly (icv) administered relaxin-3 stimulates food intake. Icv administered relaxin (identical to relaxin-2 in humans) affects the secretion of vasopressin and drinking behavior. Relaxin-3 partly binds relaxin family peptide receptor 1, which is a specific receptor to relaxin. Thus, we hypothesized that relaxin-3 would have physiological effects in the body fluid balance. However, the effects of relaxin-3 in the body fluid balance remain unknown. In the present study, we revealed that icv administered relaxin-3 induced dense Fos-like immunoreactivity (Fos-LI) in the rat hypothalamus and circumventricular organs including the organum vasculosum of the lamina terminalis, the median preoptic nucleus, supraoptic nucleus (SON), the subfornical organ (SFO) and the paraventricular nucleus (PVN), that are related to the central regulation of body fluid balance. Icv administered relaxin-3 (54, 180 and 540 pmol/rat) also induced a significant increase in c-fos gene expression in a dose-dependent manner in the SON, SFO and PVN. Further, icv administered relaxin-3 (180 pmol/rat) significantly increased water intake, and the effect was as strong as that of relaxin-2 (180 pmol/rat). These results suggest that icv administered relaxin-3 activates osmosensitive areas in the brain and plays an important role in the regulation of body fluid balance. (C) 2010 Elsevier Inc. All rights reserved.