ADOLESCENT DRINKING TARGETS CORTICOTROPIN-RELEASING FACTOR PEPTIDE-LABELED CELLS IN THE CENTRAL AMYGDALA OF MALE AND FEMALE RATS
ADOLESCENT DRINKING TARGETS CORTICOTROPIN-RELEASING FACTOR PEPTIDE-LABELED CELLS IN THE CENTRAL AMYGDALA OF MALE AND FEMALE RATS
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DOI：
10.1016/j.neuroscience.2013.04.024
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发表时间：
2013-09-26
期刊：
影响因子：
3.3
通讯作者：
Richardson, H. N.
中科院分区：
文献类型：
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作者：
Karanikas, C. A.;Lu, Y-L;Richardson, H. N.
青春期是一个发育期，许多青少年第一次喝酒，经常酗酒。早发性酒精与成年期压力相关疾病的风险增加有关，这表明酒精可能会干扰压力调节系统的发育。我们研究了自愿酒精暴露对青春期雄性和雌性大鼠杏仁核中央核（CeA）促肾上腺皮质激素释放因子（CRF）肽产生细胞的影响。这些细胞对于自主神经和对压力的行为反应很重要，与成瘾有关，并随着青少年的发展而变化。动物在青春期早期（出生后（PD）28-42天）自我施用甜味酒精，并且在PD 43获得脑用于CRF肽免疫标记。与雄性动物相比，雌性动物的CeA中CRF免疫反应（-ir）细胞较少。在雄性和雌性中，与对照条件相比，酒精自我给药减少了CeA中CRF-ir细胞的数量，在对照条件下，大鼠自我给药了同等水平的不含酒精的甜味水。在终纹床核中未观察到肽标记减少，表明这些变化具有区域特异性。杏仁核CRF细胞群的改变可能对青春期和以后的生活中对酒精和应激障碍的易感性具有重要意义。(C)2013年IBRO。由爱思唯尔有限公司出版。保留所有权利。
Adolescence is a developmental period when many teenagers first drink alcohol and often engage in binge drinking. Early onset of alcohol is linked to increased risk of stress-related disorders in adulthood in humans, suggesting that alcohol may interfere with development of the stress regulatory system. We investigated the effect of voluntary alcohol exposure on corticotropin-releasing factor (CRF) peptide-producing cells in the central nucleus of the amygdala (CeA) in adolescent male and female rats. These cells are important for the autonomic and behavioral responses to stress, have been implicated in addiction, and change over adolescent development. Animals self-administered sweetened alcohol during early adolescence (postnatal days (PDs) 28-42) and brains were obtained on PD 43 for CRF peptide immunolabeling. Females had fewer CRF immunoreactive (-ir) cells in the CeA compared to males. In both males and females, alcohol self-administration reduced the number of CRF-ir cells in the CeA compared to control conditions in which rats self-administered equivalent levels of sweetened water that did not contain alcohol. Reduced peptide labeling was not observed in the bed nucleus of the stria terminalis, indicating regional specificity of these changes. Alterations within the CRF cell population of the amygdala may have important implications for susceptibility to alcohol and stress disorders during adolescence and later on in life. (C) 2013 IBRO. Published by Elsevier Ltd. All rights reserved.