CHEMICAL CHARACTERIZATION OF NEUROENDOCRINE TARGETS FOR PROGESTERONE IN THE FEMALE RAT-BRAIN AND PITUITARY
CHEMICAL CHARACTERIZATION OF NEUROENDOCRINE TARGETS FOR PROGESTERONE IN THE FEMALE RAT-BRAIN AND PITUITARY
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DOI：
10.1159/000125350
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发表时间：
1990-03-01
影响因子：
4.1
通讯作者：
PFAFF, DW
中科院分区：
文献类型：
--
作者：
FOX, SR;HARLAN, RE;PFAFF, DW
在全身施用合成孕激素配体[3H]-R5020后，通过将免疫细胞化学与放射自显影相结合来测定雌性大鼠大脑和垂体中对孕酮作用直接反应的一些细胞类型的分泌产物。报告了四个主要发现：（1）下丘脑弓状核中大约 90% 的酪氨酸羟化酶（TH）免疫反应性神经元具有孕酮受体，而下丘脑其他部分（例如脑室周围区域和未定带）的 TH 免疫反应性神经元则没有。 (2)下丘脑中大约30%的β-内啡肽神经元具有孕酮受体。 (3)所检查的黄体生成素释放激素神经元均没有孕酮受体。 (4) 垂体前叶中大约98%具有孕激素受体的细胞含有黄体生成素。泌乳素细胞不含孕激素受体。大脑中的许多孕激素靶标仍有待化学表征。讨论了黄体酮诱导基因和神经内分泌控制系统的影响。
The secretory products of some of the cell types which respond directly to actions of progesterone in the female rat brain and pituitary were determined by combining immunocytochemistry with autoradiography following systemic administration of the synthetic progestin ligand [3H]-R5020. Four major findings are reported: (1) Approximately 90% of the tyrosine hydroxylase (TH)-immunoreactive neurons in the hypothalamic arcuate nucleus have progesterone receptors, while TH-immunoreactive neurons in other portions of the hypothalamus (e.g. the periventricular region and the zona incerta) do not. (2) Approximately 30% of the .beta.-endorphin neurons in the hypothalamus have progesterone receptors. (3) None of the luteinizing hormone-releasing hormone neurons examined have progesterone receptors. (4) Approximately 98% of the cells in the anterior pituitary that have progesterone receptors contain luteinizing hormone. Lactotrophs do not contain progesterone receptors. Many progestin targets in the brain remain to be characterized chemically. The implications for progesterone-inducible genes and neuroendocrine control systems are discussed.