The Anopheles coluzzii microbiome and its interaction with the intracellular parasite Wolbachia.
The Anopheles coluzzii microbiome and its interaction with the intracellular parasite Wolbachia.
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库氏按蚊微生物组及其与细胞内寄生虫沃尔巴克氏体的相互作用。
DOI：
10.1038/s41598-020-70745-0
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发表时间：
2020
影响因子：
4.6
通讯作者：
Neafsey,DanielE
中科院分区：
文献类型：
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作者：
Straub,TimothyJ;Shaw,WRobert;Marcenac,Perrine;Sawadogo,SimonP;Dabiré,RochK;Diabaté,Abdoulaye;Catteruccia,Flaminia;Neafsey,DanielE
沃尔巴克氏菌是一种内共生的α -变形菌，常见于昆虫中，可以抑制蚊子传播人类病原体。生物防治项目正在进行中，使用经非自然沃尔巴克氏杆菌感染的埃及伊蚊来减少登革热病毒的传播。沃尔巴克氏体对疟蚊的生物学和传播能力的影响知之甚少。疟蚊是疟疾寄生虫的传播媒介。一种自然产生的沃尔巴克氏体菌株wAnga感染了布基纳法索主要疟疾媒介冈比亚疟蚊和科鲁兹疟蚊。以往研究发现wanga感染与蚊子疟原虫感染呈负相关，并且wanga影响蚊子产卵行为。在这里，我们调查了安自然种群中的wanga。coluzzii及其与其他常驻微生物群的相互作用使用靶向16S测序。虽然我们没有发现与wanga感染相关的微生物群组成有重大差异，但我们确实发现了一些与产卵后雌蚊中wanga存在或不存在相关的分类群，但需要注意的是，由于wanga对产卵时间的意外影响，我们不能排除批次效应。这些数据表明wanga可能影响或与按蚊微生物群相互作用，这可能有助于wanga对按蚊生物学和媒介能力的影响。
Wolbachia, an endosymbiotic alpha-proteobacterium commonly found in insects, can inhibit the transmission of human pathogens by mosquitoes. Biocontrol programs are underway usingAedes aegyptimosquitoes trans-infected with a non-naturalWolbachiastrain to reduce dengue virus transmission. Less is known about the impact ofWolbachiaon the biology and vectorial capacity ofAnophelesmosquitoes, the vectors of malaria parasites. A naturally occurring strain ofWolbachia,wAnga, infects populations of the major malaria vectorsAnopheles gambiaeandAnopheles coluzziiin Burkina Faso. Previous studies foundwAnga infection was negatively correlated withPlasmodiuminfection in the mosquito andwAnga influenced mosquito egg-laying behavior. Here, we investigatewAnga in natural populations ofAn. coluzziiand its interactions with other resident microbiota using targeted 16S sequencing. Though we find no major differences in microbiota composition associated withwAnga infection, we do find several taxa that correlate with the presence or absence ofwAnga in female mosquitoes following oviposition, with the caveat that we could not rule out batch effects due to the unanticipated impact ofwAnga on oviposition timing. These data suggestwAnga may influence or interact with theAnophelesmicrobiota, which may contribute to the impact ofwAnga onAnophelesbiology and vectorial capacity.