Phosphonic Acid Functionalized Periodic Mesoporous Organosilicas and Their Potential Applications in Selective Enrichment of Phosphopeptides
Phosphonic Acid Functionalized Periodic Mesoporous Organosilicas and Their Potential Applications in Selective Enrichment of Phosphopeptides
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DOI：
10.1021/jp8093534
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发表时间：
2009-01
影响因子：
3.7
通讯作者：
Peiyuan Wang;Liang Zhao;R. Wu;Hua Zhong;Hanfa Zou;Jie Yang;Qihua Yang
中科院分区：
文献类型：
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作者：
Peiyuan Wang;Liang Zhao;R. Wu;Hua Zhong;Hanfa Zou;Jie Yang;Qihua Yang
以Brij-76为模板剂，在酸性介质中以1,2-二（三甲氧基硅基）乙烷和二氧基磷乙基三乙氧基硅烷共缩合制备了膦酸功能化的周期性介孔有机硅。结构表征表明，在无机盐NaCl的存在下，可以得到二维六边形介孔材料。透射电镜结果表明，当NaCl/Brij-76质量比为3和4时，纳米通道中存在大量的结构缺陷孔。将金属离子（Zr4+和Fe3+）在介孔中与磷酸配位后，作为潜在的固定化金属亲和层析吸附剂用于磷酸肽的选择性富集。由于配位金属离子与磷酸肽的磷基之间具有较强的亲和相互作用，具有较高的比表面积和独特的介孔结构，从而使磷酸的捕获更加容易。
Phosphonic acid functionalized periodic mesoporous organosilicas were synthesized by co-condensation of 1,2-bis(trimethoxysilyl)ethane and diethoxyphosphorylethyl-triethoxysilane in acidic medium using Brij-76 as a template. Structural characterizations showed that the mesoporous materials with 2-D hexagonal mesostructures could be obtained in the presence of an inorganic salt, NaCl. The results of transmission electron microscopy revealed that the materials synthesized with NaCl/Brij-76 mass ratios of 3 and 4 had extensive structural defect holes in the nanochannels. After coordinating metal ions (Zr4+ and Fe3+) with phosphonic acid in the mesopore, the materials were applied as the potential immobilized metal affinity chromatographic adsorbent for the selective enrichment of phosphopeptides. Because of the stronger affinity interaction between the coordinated metal ions and the phosphoryl groups of phosphopeptides, the higher surface area, and the unique mesoporous structure, the capture of the phosphop...