Identification, physical map location and sequence of the denV gene from bacteriophage T4.
Identification, physical map location and sequence of the denV gene from bacteriophage T4.
复制标题
噬菌体 T4 中 denV 基因的鉴定、物理图谱位置和序列。
DOI：
10.1093/nar/12.21.8085
复制
发表时间：
1984
影响因子：
14.9
通讯作者：
J. K. Deriel
中科院分区：
文献类型：
--
作者：
Kristoffer Valerie;E. Henderson;J. K. Deriel
来自噬菌体T4的denV基因，其编码核酸内切酶V，一种小的DNA修复酶，已经通过DNA测序和遗传学相结合的方法被克隆和鉴定，独立于克隆基因的表型效应。对适当的DenV+和DenV-缺失突变体进行物理作图，以精确定义包含denV基因的区域。对该区域进行测序以鉴定denV基因的正确大小的蛋白质编码序列（400-500 bp）。最后，通过对从四个遗传和表型良好表征的denV突变体克隆的相应片段进行测序来确认鉴定。denV基因位于T4基因组上的64 kb处，与ipII基因相邻，并编码138个氨基酸的碱性蛋白质，推断分子量为16，078。
The denV gene from bacteriophage T4, which codes for endonuclease V, a small DNA repair enzyme, has been cloned and identified by an approach combining DNA sequencing and genetics, independent of the phenotypic effect of the cloned gene. Appropriate DenV+ and DenV- deletion mutants were mapped physically to define precisely a region encompassing the denV gene. This region was sequenced in order to identify a protein-coding sequence of the correct size for the denV gene (400-500 bp). Finally, identification was confirmed by sequencing the corresponding fragments cloned from four genetically and phenotypically well-characterized denV mutants. The denV gene is located at 64 kb on the T4 genome, adjacent to the ipII gene, and codes for a basic protein of 138 amino acids with a deduced molecular weight of 16,078.