Analytical assays of human HSP27 and thermal-stress survival of Escherichia coli cells that overexpress it.
Analytical assays of human HSP27 and thermal-stress survival of Escherichia coli cells that overexpress it.
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DOI：
10.1016/j.bbrc.2006.01.090
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发表时间：
2006-03
影响因子：
3.1
通讯作者：
S. Takeuchi
中科院分区：
文献类型：
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作者：
S. Takeuchi
HSP27 是一种小热休克蛋白 (sHSP)。所有生物体都会产生此类蛋白质。这些小的 HSP 表现出类似分子伴侣的活性，可以与未折叠的多肽结合并防止体外不受控制的蛋白质聚集。细胞抗凋亡功能和细胞存活率的提高与 HSP 表达的增加相关。本研究提出了使用大肠杆菌表达系统的细胞的热应激生存模型，其中人类 HSP27（一种重组蛋白）是可诱导的。结果表明，过表达人 HSP27 的大肠杆菌细胞对 50°C 热应激的耐受性增强。
HSP27 is a small heat-shock protein (sHSP). Such proteins are produced in all organisms. These small HSPs exhibit chaperone-like activity that can bind to unfolded polypeptides and prevent uncontrolled protein aggregation in vitro. Cellular anti-apoptosis function and enhanced cell survival are correlated with increased expression of HSPs. This study presents a thermal-stress survival model for cells using the Escherichia coli expression system for which human HSP27, a recombinant protein, is inducible. Results show that E. coli cells overexpressing human HSP27 have enhanced tolerance to 50°C thermal stress.