Modulation of Lon protease activity and aconitase turnover during aging and oxidative stress
Modulation of Lon protease activity and aconitase turnover during aging and oxidative stress
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DOI：
10.1016/s0014-5793(02)03638-4
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发表时间：
2002-12-04
期刊：
影响因子：
3.5
通讯作者：
Davies, KJA
中科院分区：
文献类型：
--
作者：
Bota, DA;Van Remmen, H;Davies, KJA
我们比较了幼龄和老年、野生型和Sod2(-/+)杂合子小鼠骨骼肌中Lon蛋白酶的表达，并研究了Lon在受损(氧化)蛋白积累中的作用。在衰老和氧化挑战的动物中，Lon蛋白的水平较低，这种Lon缺乏与碳化蛋白水平的增加有关。我们鉴定了其中一个是乌头酸酶，另一个是乌头酸酶的裂解产物，我们也可以在体外用过氧化氢处理纯化的乌头酸酶来产生它。这些结果表明，衰老和氧化应激下调Lon蛋白酶的表达，进而可能导致受损蛋白质在线粒体内的积累，如乌头酸酶。(C)2002年，由Elsevier Science B.V.代表欧洲生化学会联合会出版。
We compared Lon protease expression in murine skeletal muscle of young and old, wild-type and Sod2(-/+) heterozygous mice, and studied Lon involvement in the accumulation of damaged (oxidized) proteins. Lon protease protein levels were lower in old and oxidatively challenged animals, and this Lon deficiency was associated with increased levels of carbonylated proteins. We identified one of these proteins as aconitase, and another as an aconitase fragmentation product, which we can also generate in vitro by treating purified aconitase with H2O2. These results imply that aging and oxidative stress down-regulate Lon protease expression which, in turn, may be responsible for the accumulation of damaged proteins, such as aconitase, within mitochondria. (C) 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.