Criteria for a proof of migration routes of immature parasites inside hosts exemplified by studies of Strongyloides ratti in the rat
Criteria for a proof of migration routes of immature parasites inside hosts exemplified by studies of Strongyloides ratti in the rat
复制标题
以大鼠体内的鼠类圆线虫研究为例，证明宿主体内未成熟寄生虫迁移途径的标准
DOI：
10.1017/s0031182000080185
复制
发表时间：
1988
期刊：
影响因子：
2.4
通讯作者：
P. A. G. Wilson
中科院分区：
文献类型：
--
作者：
N. R. Tindall;P. A. G. Wilson
总结第一个严格的证明适用于迁移途径的感染性少年大型寄生虫在其主机。第三阶段幼虫的同源菌株的圆线虫ratti适用于准确的剂量小于20的皮肤侧的年轻大鼠，16-40小时后，在鼻额部的头部。在48只动物中，该部位20 - 25 h之间回收的剂量峰比例的平均值为0.316 ± 0.021。在40只同时感染的其他大鼠中，到达小肠的剂量的平均比例至少为0.837 ± 0.013。证明在于验证不等式> 1。通过适当的统计检验，这两个比例的平均值之和超过1的概率为1/3.5 × 106。因此，可以有效地确定，头部的鼻额部分是这种寄生虫从皮肤到宿主肠道的至少一条路径的一部分。通过适当保护感染部位，证实了通过内部途径迁移至头部，而不是梳理的结果。在15-40 h期间，从相同大鼠的颅骨中回收到了幼虫，但剂量的峰值比例出现在20 h，并且<在该位置。因此，颅骨是否也是通路的一部分仍然没有确定。讨论了这种新型分析在感染阶段宿主内迁移的一般背景下的重要性，并得出结论，在将其应用于其他作者的数据集之后，唯一可以证明证据的案例是大鼠的前头骨。S。ratti（现有数据）和巴西日本圆线虫同一宿主的肺（Twohy，1956）。
SUMMARY The first rigorous proof applicable to the migration pathway of an infective juvenile macroparasite inside its host is presented. Third-stage larvae of a homogonic strain of Strongyloides ratti applied in exact doses of less than 20 to the skin of the flank of young rats were recovered 16–40 h later in the naso-frontal part of the head. The peak proportion of the dose recovered between 20 and 25 h in this site had a mean value of 0·316 ± 0·021 in 48 animals. In 40 other rats infected simultaneously the mean proportion of the dose that reached the small intestine was at least 0·837+0·013. Proof resides in verification of the inequality > 1. With appropriate statistical tests the excess of the sum of the means of these two proportions over unity is shown to have a probability of occurring by chance of 1 in 3·5 × 106. Thus it is effectively certain that the naso-frontal portion of the head is part of at least one pathway taken by this parasite on its way from the skin to the intestine of its host. By suitable protection of the infection site it was confirmed that migration to the head was achieved by an internal route and not as a result of grooming. Larvae were recovered from the cranium in the same rats over the period 15–40 h, but the peak proportion of the dose occurred at 20 h, and < in this location. Whether the cranium is also part of the pathway is therefore still undecided. The significance of this novel analysis in the general context of in-host migration of infective stages is discussed and it is concluded, following its application to data sets from other authors, that the only cases in which proof can be demonstrated are the anterior skull of the rat for S. ratti (present data) and the lung of the same host for Nippostrongylus brasiliensis (Twohy, 1956).
鼠类圆线虫：将大鼠的保护性免疫分解为全身和肠道成分。
影响因子：
2.1
作者：
Bell,RG;Adams,LS;Gerb,J
通讯作者：
Gerb,J