Activated platelets enhance microparticle formation and platelet-leukocyte interaction in severe trauma and sepsis
Activated platelets enhance microparticle formation and platelet-leukocyte interaction in severe trauma and sepsis
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DOI：
10.1097/00005373-200105000-00005
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发表时间：
2001-05-01
影响因子：
--
通讯作者：
Sugimoto, H
中科院分区：
文献类型：
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作者：
Ogura, H;Kawasaki, T;Sugimoto, H
背景：最近有报道称活化血小板可产生血小板微粒并增强血小板-白细胞相互作用。血小板在全身炎症反应综合征（SIRS）中的确切作用尚未阐明。本研究的目的是评估微粒形成酸血小板-白细胞相互作用在严重创伤和败血症。方法：26例严重SIRS患者（SIRS标准和血清C-反应蛋白> 10 mg/dL）和12名健康志愿者进行了研究。采用流式细胞术检测在有或无离子霉素、N-甲酰甲硫氨酰-亮氨酰-苯丙氨酸或抗CD 62 β单克隆抗体的情况下微粒形成、血小板P-选择素表达、血小板-单核细胞结合和血小板-多形核白细胞（PMNL）结合。可溶性P-选择素，血栓调节蛋白，新蝶呤，PMNL弹性蛋白酶在血液中也measured.Results：微粒形成，P-选择素表达血小板，血小板-单核细胞结合或不结合离子霉素，和血小板-PMNL结合离子霉素显着增加严重的SIRS患者在正常志愿者的值相比。严重SIPS患者血小板-白细胞结合的增加被P-选择素阻断剂显著抑制，而不被N-甲酰甲硫氨酰亮氨酰苯丙氨酸增强，这些患者血液中可溶性P-选择素、血栓调节蛋白、新蝶呤和PMNL弹性蛋白酶也增加。活化血小板增强严重创伤和脓毒症中微粒形成和血小板-白细胞相互作用，增强的血小板-白细胞相互作用依赖于P-选择素的表达，并可能参与严重炎症损伤后的全身炎症反应。
Background: activated platelets have been recently reported to produce platelet microparticles and to enhance platelet-leukocyte interaction. The precise role of platelets in systemic inflammatory response syndrome (SIRS) has not been clarified. The objective of this study was to evaluate microparticle formation acid platelet-leukocyte interaction in severe trauma and sepsis.Methods: Twenty-six patients with severe SIRS (SIRS criteria and serum C-reactive protein > 10 mg/dL) and 12 healthy volunteers were studied. The severe SIRS was caused by trauma in 12 patients and sepsis in 14, Microparticle formation, P-selectin expression on platelets, platelet-monocyte binding, and platelet-polymorphonuclear leukocyte (PMNL) binding were measured by flow cytometry in the presence or absence of ionomycin, N-formyl-methionyl-leucyl-phenylalanine, or anti-CD62p monoclonal antibody. Soluble P-selectin, thrombomodulin, neopterin, and PMNL elastase in blood were also measured.Results: Microparticle formation, P-selectin expression on platelets, platelet-monocyte binding with or without ionomycin, and platelet-PMNL binding with ionomycin significantly increased in patients with severe SIRS in comparison with values in normal volunteers. The increased platelet-leukocyte binding in severe SIPS patients was markedly inhibited by P-selectin blockade and was not enhanced by N-formyl-methionyl-leucyl-phenylalanine, Soluble P-selectin, thrombomodulin, neopterin, and PMNL elastase in blood also increased in these patients.Conclusion: Activated platelets enhance microparticle formation and platelet-leukocyte interaction in severe trauma and sepsis, Enhanced platelet-leukocyte interaction is dependent on P-selectin expression and may be involved in the systemic inflammatory response after severe inflammatory insult.