Vibrio fujianensis sp nov., isolated from aquaculture water
Vibrio fujianensis sp nov., isolated from aquaculture water
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DOI：
10.1099/ijsem.0.002642
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发表时间：
2018-04-01
影响因子：
2.8
通讯作者：
Wang, Duochun
中科院分区：
文献类型：
--
作者：
Fang, Yujie;Chen, Aiping;Wang, Duochun
从福建省养殖水体中分离到一株革兰氏染色阴性兼性厌氧菌株，命名为FJ201301(T)。16S rRNA基因序列的系统发育分析表明，菌株FJ201301(T)属于弧菌属，与辛辛纳提弧菌ATCC 35912(T)形成一个独立的聚类，与salilacus弧菌CGMCC 1.12427 (T)相似性最高。FJ201301(T)在16S rRNA基因上发现了一个15 bp的插入，这是区分菌株FJ201301(T)与几个系统发育上的邻居（如辛辛纳提菌）的重要标记。对8个基因（ftsZ、gapA、gyrB、mreB、pyrH、recA、rpoA和topA，串联4135 bp序列）的多位点序列分析表明，菌株FJ201301 T与V. salilacus CGMCC 1.12427(T)、V. cinnatiensis ATCC 35912 (T)、Vibrio injenensis KCTC 32233(T)和Vibrio metschnikovii CIP 69.14(T)聚在一起，形成了一个独立的长系统发育分支。此外，菌株FJ201301(T)与V.辛辛纳提ensis ATCC 35912(T)之间的硅DNA-DNA杂交和平均核苷酸一致性值最高，分别为26.3%和83.1%，低于物种划分的截止水平，分别为70%和95%。生化、序列和基因组分析表明，菌株FJ201301(T)为弧菌属新种，建议命名为福建弧菌（Vibrio fujianensis sp. 11 .）。型应变为FJ201301(T) (= DSM 104687（T) = CGMCC 1.16099(T)）。
A Gram-stain-negative, facultatively anaerobic strain, designated FJ201301(T), was isolated from aquaculture water collected from Fujian province, China. Phylogenetic analysis of 16S rRNA gene sequences indicated that strain FJ201301(T) belonged to the genus Vibrio, formed a distinct cluster with Vibrio cincinnatiensis ATCC 35912(T) and shared the highest similarity with Vibrio salilacus CGMCC 1.12427 (T). A 15 bp insertion found in the 16S rRNA gene was a significant marker that distinguished strain FJ201301(T) from several phylogenetic neighbours (e. g. V. cincinnatiensis). Multilocus sequence analysis of eight genes (ftsZ, gapA, gyrB, mreB, pyrH, recA, rpoA and topA; concatenated 4135 bp sequence) showed that, forming a long and independent phylogenetic branch, strain FJ201301 T clustered with V. cincinnatiensis ATCC 35912 (T), Vibrio injenensis KCTC 32233(T) and Vibrio metschnikovii CIP 69.14(T) clearly separated from V. salilacus CGMCC 1.12427(T). Furthermore, the highest in silico DNA-DNA hybridization and average nucleotide identity values between strain FJ201301(T) and the closest related species were 26.3 and 83.1% with V. cincinnatiensis ATCC 35912(T), less than the proposed cutoff levels for species delineation, i. e. 70 and 95 %, respectively. Biochemical, sequence and genomic analysis suggested the designation of strain FJ201301(T) representing a novel species of the genus Vibrio, for which the name Vibrio fujianensis sp. nov. is proposed. The type strain is FJ201301(T) (= DSM 104687(T) = CGMCC 1.16099(T)).