CIRCUMSPOROZOITE PROTEIN HETEROGENEITY IN THE HUMAN MALARIA PARASITE PLASMODIUM-VIVAX
CIRCUMSPOROZOITE PROTEIN HETEROGENEITY IN THE HUMAN MALARIA PARASITE PLASMODIUM-VIVAX
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DOI：
10.1126/science.2672336
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发表时间：
1989-09-01
期刊：
影响因子：
56.9
通讯作者：
PRASITTISUK, C
中科院分区：
文献类型：
--
作者：
ROSENBERG, R;WIRTZ, RA;PRASITTISUK, C
表型异质性的重复部分的人疟疾环子孢子（CS）蛋白，候选疫苗的主要目标，已被found. Over 14%的简单间日疟原虫疟疾在泰国西部的两个网站的临床病例产生的子孢子免疫学上不同于以前的特点的物种的例子。抗其他间日疟原虫分离株CS蛋白和抗其他人类和猿类疟疾种属的CS蛋白的单克隆抗体不与这些非反应性子孢子结合，用新世界CS蛋白重复部分制成的候选疫苗免疫的猴子的抗体也不与这些非反应性子孢子结合。非反应性分离物的保守区域I和II之间的CS蛋白基因的部分含有九肽重复序列，Ala-Asn-Gly-Ala-Gly-Asn-Gln-Pro-Gly，与已发表的九肽序列仅在三个氨基酸位置相同。这种异质性意味着基于一个分离株的CS蛋白重复序列的间日疟原虫疫苗不会具有普遍保护性。
Phenotypic heterogeneity in the repetitive portion of a human malaria circumsporozoite (CS) protein, a major target of candidate vaccines, has been found. Over 14% of clinical cases of uncomplicated Plasmodium vivax malaria at two sites in western Thailand produced sporozoites immunologically distinct from previously characterized examples of the species. Monoclonal antibodies to the CS protein of other P. vivax isolates and to other species of human and simian malarias did not bind to these nonreactive sporozoites, nor did antibodies from monkeys immunized with a candidate vaccine made from the repeat portion of a New World CS protein. The section of the CS protein gene between the conserved regions I and II of a nonreactive isolate contained a nonapeptide repeat, Ala-Asn-Gly-Ala-Gly-Asn-Gln-Pro-Gly, identical at only three amino acid positions with published nonapeptide sequences. This heterogeneity implies that a P. vivax vaccine based on the CS protein repeat of one isolate will not be universally protective.