Molecular cloning and immunolocalization of a novel vertebrate trp homologue from Xenopus
Molecular cloning and immunolocalization of a novel vertebrate trp homologue from Xenopus
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DOI：
10.1042/0264-6021:3400593
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发表时间：
1999-06-15
影响因子：
4.1
通讯作者：
Bootman, MD
中科院分区：
文献类型：
--
作者：
Bobanovic, LK;Laine, M;Bootman, MD
我们报道了一种新的非洲爪哇瞬时受体潜力(Trp)同源物Xtrp的序列、结构和分布，该同源物是通过筛选卵母细胞cDNA文库确定的。根据序列相似性和预测的结构，Xtrp似乎是哺乳动物trp1蛋白的同源物。两种针对Xtrp序列不同区域的多克隆抗体表明，Xtrp在非洲爪哇不同组织中表达，并定位于非洲爪哇卵母细胞和HeLa细胞的质膜上。由于非洲爪哇卵母细胞的容量性钙进入之前已经被trp1反义寡核苷酸显著抑制[Tomita，Kaneko，Funayama，Kondo，Satoh和Akaike(1998)Neurosci]。让我们来吧。248,195-198]我们认为Xtrp可能是非洲爪哇组织中电容性钙离子进入的基础。
We report the sequence, structure and distribution of a novel transient receptor potential (trp) homologue from Xenopus, Xtrp, determined by screening an oocyte cDNA library. On the basis of sequence similarity and predicted structure, Xtrp appears to be a homologue of mammalian trp1 proteins. Two polyclonal antibodies raised against distinct regions of the Xtrp sequence revealed Xtrp expression in various Xenopus tissues, and the localization of Xtrp at the plasma membrane of Xenopus oocytes and HeLa cells. Since capacitative calcium entry into Xenopus oocytes has been shown previously to be substantially inhibited by trp1 antisense oligonucleotides [Tomita, Kaneko, Funayama, Kondo, Satoh and Akaike (1998) Neurosci. Lett. 248, 195-198] we suggest that Xtrp may underlie capacitative calcium entry in Xenopus tissues.