Vibriosis resistance induced by glucan treatment in tiger shrimp (Penaeus monodon)
Vibriosis resistance induced by glucan treatment in tiger shrimp (Penaeus monodon)
复制标题
虎虾（斑节对虾）葡聚糖处理诱导的弧菌抗性
作者：
H. Sung;G. Kou;Yen
将幼体后的虎虾（斑节对虾）浸入充气的β-葡聚糖悬浮液中3小时。在浸于浓度为0.5、1和2 mg/ml的葡聚糖中的虾中观察到生长增强，但在浓度为0.25 mg/ml时没有观察到。虾鳃组织在浸入2 mg/ml悬浮液后立即皱缩。β-葡聚糖处理后第10、18和43天，将虾浸入含有浓度为5×107 CFU/ml的创伤弧菌细胞的细菌悬浮液中12小时，密度为每升25只;实验感染后一个月记录死亡情况。在用0.5和1 mg/ml葡聚糖处理的虾中观察到葡聚糖处理的保护作用，但在用0.25和2 mg/ml葡聚糖处理的虾中没有观察到。保护作用持续到浸泡后第18天。在体外实验中，β-葡聚糖处理增强了对虾血细胞中酚氧化酶的活性。因此，β-葡聚糖似乎是虾的短期免疫刺激剂。
Postlarval tiger shrimp (Penaeus monodon) were immersed in aerated beta-glucan suspensions for 3 h. Enhanced growth was observed in shrimp immersed in glucan at concentrations of 0.5, 1, and 2 mg/ml, but not at 0.25 mg/ml. Shrimp gill tissue became shrunken immediately after immersion in the 2 mg/ml suspension. On days 10, 18, and 43 after beta-glucan treatment, shrimp were immersed in a bacterial suspension containing Vibrio vulnificus cells at a concentration of 5×107 CFU/ml for 12 h at a density of 25 individuals per liter; deaths were recorded for one month following experimental infection. The protective effect of glucan treatment was observed in those shrimp treated with 0.5 and 1 mg/ml glucan, but not in shrimp treated with 0.25 and 2 mg/ml glucan. The protective effect lasted until day 18 following immersion. In vitro, beta-glucan treatment enhanced the phenoloxidase activity in shrimp hemocytes. It therefore appears that beta-glucan is a short-term immunostimulant for shrimp.