Pre- and postnatal development of differentiated functions in rat intestinal epithelial cells.
Pre- and postnatal development of differentiated functions in rat intestinal epithelial cells.
复制标题
大鼠肠上皮细胞出生前和出生后分化功能的发育。
DOI：
10.1016/0012-1606(85)90483-x
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发表时间：
1985
影响因子：
2.7
通讯作者：
Quaroni,A
中科院分区：
文献类型：
--
作者：
Quaroni,A
一组针对肠细胞表面成分的单克隆抗体被用来比较胚胎、哺乳和成年大鼠小肠上皮细胞中分化特异性抗原的表达。间接免疫荧光染色和洗涤剂溶解膜蛋白的免疫纯化，以及单和二维平板凝胶电泳分析表明，胎儿肠细胞（妊娠第21天）表达了大多数成人吸收绒毛细胞典型的分化特异性标记。在哺乳大鼠肠道不同绒毛细胞群中，抗原表达存在明显的异质性，并鉴定出三种细胞表面成分，这些成分只存在于肠道发育时期。隐窝和绒毛细胞中抗原表达模式的显著变化，以及大多数管腔膜成分的表观等电点的变化，与断奶时肠黏膜的成熟有关。新生大鼠注射可的松不能过早诱导这些变化，提示糖皮质激素以外的因素对出生后肠上皮的发育起作用。这些结果表明，肠上皮细胞在产前和产后成熟的不同阶段在生物学特性和调控机制上存在基本差异。
A panel of monoclonal antibodies to intestinal cell surface components has been used to compare the expression of differentiation-specific antigens in the epithelial cells of fetal, suckling, and adult rat small intestine. Indirect immunofluorescence staining, and immunopurification of detergent-solubilized membrane proteins, followed by single- and two-dimensional slab gel electrophoretic analysis, have demonstrated that fetal intestinal cells (at day 21 of gestation) express most differentiation-specific markers typical of adult absorptive villus cells. A marked heterogeneity in antigen expression was observed among different villus cell populations in suckling rat intestine, and three cell surface components were identified which are exclusively present during this period of intestinal development. STriking changes in the patterns of antigen expression in crypt and villus cells, and variations in the apparent isoelectric points for most luminal membrane components, were associated with the maturation of the intestinal mucosa at weaning. These changes could not be prematurely induced by cortisone injection in newborn rats, suggesting that factors other than glucocorticoids are responsible for the postnatal development of the intestinal epithelium. These results suggest that basic differences in biological properties and regulatory mechanisms exist among intestinal epithelial cells at different stages of pre- and postnatal maturation.