Excitation-emission matrix (EEM) fluorescence spectroscopy for characterization of organic matter in membrane bioreactors: Principles, methods and applications
Excitation-emission matrix (EEM) fluorescence spectroscopy for characterization of organic matter in membrane bioreactors: Principles, methods and applications
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用于表征膜生物反应器中有机物的激发-发射矩阵 (EEM) 荧光光谱：原理、方法和应用
DOI：
10.1007/s11783-019-1210-8
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发表时间：
2020-01
影响因子：
6.4
通讯作者：
Xia Huang
中科院分区：
文献类型：
--
作者：
Jinlan Yu;Kang Xiao;Wenchao Xue;Yue-xiao Shen;Jihua Tan;Shuai Liang;Yanfen Wang;Xia Huang
膜生物反应器（MBR）技术是污水处理领域的一颗冉冉升起的星星。膜生物反应器的污染物去除和膜污染性能与系统中的溶解性有机物（DOM）密切相关。三维激发发射矩阵（3D-EEM）荧光光谱，一个强大的工具，快速和灵敏的表征DOM，已被广泛应用于MBR的研究，但是，只有有限的一部分EEM指纹信息被利用。本文回顾了荧光EEM的原理和方法，综述了EEM在MBR研究中表征DOM的最新进展。系统介绍了从EEM中提取的荧光峰位置/强度、波长区域分布和光谱解卷积（给出荧光组分负荷/分数）信息，并讨论了如何利用这些信息来解释MBR系统中DOM的化学组成、理化性质、生物活性、膜截留/污染行为和迁移/转化命运。除了传统的EEM指标，新的荧光参数总结了潜在的用途，包括量子产率，斯托克斯位移，激发能态，和荧光寿命。目前的局限性，EEM为基础的DOM表征进行了讨论，提出了可能的措施，以提高MBR监测的应用。
The membrane bioreactor (MBR) technology is a rising star for wastewater treatment. The pollutant elimination and membrane fouling performances of MBRs are essentially related to the dissolved organic matter (DOM) in the system. Three-dimensional excitation-emission matrix (3D-EEM) fluorescence spectroscopy, a powerful tool for the rapid and sensitive characterization of DOM, has been extensively applied in MBR studies; however, only a limited portion of the EEM fingerprinting information was utilized. This paper revisits the principles and methods of fluorescence EEM, and reviews the recent progress in applying EEM to characterize DOM in MBR studies. We systematically introduced the information extracted from EEM by considering the fluorescence peak location/ intensity, wavelength regional distribution, and spectral deconvolution (giving fluorescent component loadings/scores), and discussed how to use the information to interpret the chemical compositions, physiochemical properties, biological activities, membrane retention/fouling behaviors, and migration/ transformation fates of DOM in MBR systems. In addition to conventional EEM indicators, novel fluorescent parameters are summarized for potential use, including quantum yield, Stokes shift, excited energy state, and fluorescence lifetime. The current limitations of EEM-based DOM characterization are also discussed, with possible measures proposed to improve applications in MBR monitoring.
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影响因子：
2.7
作者：
Kothawala, Dolly N.;Murphy, Kathleen R.;Tranvik, Lars J.
通讯作者：
Tranvik, Lars J.
影响因子：
12.8
作者：
L. Deng;H. Ngo;Wenshan Guo;Hongwei Zhang
通讯作者：
L. Deng;H. Ngo;Wenshan Guo;Hongwei Zhang
影响因子：
3.7
作者：
M. Timmerman
通讯作者：
M. Timmerman
使用正面激发发射矩阵 (FF-EEM) 光谱表征超滤膜 FA 上的荧光污垢：污垢演变和机理分析
影响因子：
12.8
作者：
Yu Huarong;Wu Zijian;Zhang Xiaolei;Qu Fangshu;Wang Peng;Liang Heng
通讯作者：
Liang Heng
DOI：
10.3390/s120100972
发表时间：
2012
期刊：
Sensors (Basel, Switzerland)
影响因子：
--
作者：
Hur J;Cho J
通讯作者：
Cho J