Light-induced Variation in Phenolic Compounds in Cabernet Sauvignon Grapes (Vitis vinifera L.) Involves Extensive Transcriptome Reprogramming of Biosynthetic Enzymes, Transcription Factors, and Phytohormonal Regulators.
Light-induced Variation in Phenolic Compounds in Cabernet Sauvignon Grapes (Vitis vinifera L.) Involves Extensive Transcriptome Reprogramming of Biosynthetic Enzymes, Transcription Factors, and Phytohormonal Regulators.
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DOI：
10.3389/fpls.2017.00547
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发表时间：
2017
影响因子：
5.6
通讯作者：
Wang J
中科院分区：
文献类型：
--
作者：
Sun RZ;Cheng G;Li Q;He YN;Wang Y;Lan YB;Li SY;Zhu YR;Song WF;Zhang X;Cui XD;Chen W;Wang J
光环境对葡萄（葡萄属vinifera L.）浆果的发育和酚类化合物的生物合成，并最终影响葡萄酒的质量。在这里，羟基肉桂酸（HCAs）和黄酮类化合物的积累和组成的变化，以及全球基因表达的赤霞珠葡萄浆果在不同的物候期阳光照射处理进行了分析。不同季节光照对果皮黄烷-3-醇和花色苷的积累影响不一致，但在成熟期和采收期显著增加了果皮中HCA的含量，并随着光照时间和光照强度的增加，果皮中黄烷-3-醇和花色苷的积累显著增加。如在阳光暴露的浆果中，在编码苯丙氨酸解氨酶、4-香豆酸的基因的表达之间观察到高度显著的相关性：CoA连接酶、黄烷酮3-羟化酶和黄酮醇合酶家族成员以及酚类生物合成途径中相应的代谢物积累，其可由MYB、bHLH、WRKY、AP 2/EREBP、C2 C2、NAC、和C2 H2转录因子（TF）。此外，还鉴定了一些生长素、乙烯和脱落酸信号转导所需的候选基因，这些基因可能参与了浆果发育和类黄酮生物合成对增强阳光照射的响应。综上所述，本研究对光诱导的葡萄果实酚类物质代谢和转录组变化，特别是转铁蛋白和植物激素信号组分的动态响应提供了有价值的综述，有助于进一步了解葡萄果实响应阳光的酚类物质生物合成调控。
Light environments have long been known to influence grape (Vitis vinifera L.) berry development and biosynthesis of phenolic compounds, and ultimately affect wine quality. Here, the accumulation and compositional changes of hydroxycinnamic acids (HCAs) and flavonoids, as well as global gene expression were analyzed in Cabernet Sauvignon grape berries under sunlight exposure treatments at different phenological stages. Sunlight exposure did not consistently affect the accumulation of berry skin flavan-3-ol or anthocyanin among different seasons due to climatic variations, but increased HCA content significantly at véraison and harvest, and enhanced flavonol accumulation dramatically with its timing and severity degree trend. As in sunlight exposed berries, a highly significant correlation was observed between the expression of genes coding phenylalanine ammonia-lyase, 4-coumarate: CoA ligase, flavanone 3-hydroxylase and flavonol synthase family members and corresponding metabolite accumulation in the phenolic biosynthesis pathway, which may positively or negatively be regulated by MYB, bHLH, WRKY, AP2/EREBP, C2C2, NAC, and C2H2 transcription factors (TFs). Furthermore, some candidate genes required for auxin, ethylene and abscisic acid signal transductions were also identified which are probably involved in berry development and flavonoid biosynthesis in response to enhanced sunlight irradiation. Taken together, this study provides a valuable overview of the light-induced phenolic metabolism and transcriptome changes, especially the dynamic responses of TFs and signaling components of phytohormones, and contributes to the further understanding of sunlight-responsive phenolic biosynthesis regulation in grape berries.