Plasmodium falciparum histidine-rich protein 2-based immunocapture diagnostic assay for malaria:: Cross-reactivity with rheumatoid factors
Plasmodium falciparum histidine-rich protein 2-based immunocapture diagnostic assay for malaria:: Cross-reactivity with rheumatoid factors
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DOI：
10.1128/jcm.38.3.1184-1186.2000
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发表时间：
2000-03-01
影响因子：
9.4
通讯作者：
Rab, A
中科院分区：
文献类型：
--
作者：
Iqbal, J;Sher, A;Rab, A
最近推出的快速非显微镜免疫捕获检测疟疾感染的诊断正在评估其灵敏度和特异性在各种流行病学设置。恶性疟原虫富组氨酸蛋白2（PfHRP-2）为基础的测定（ICT）和疟原虫特异性乳酸脱氢酶试验（OptiMAL）的特异性进行了评估，在不同组的患者谁测试阴性疟疾感染显微镜。患者选自不同的疾病组：类风湿性关节炎、丙型肝炎、弓形虫病、血吸虫病和包虫病。225例患者中有133例为类风湿因子阳性。133例类风湿因子阳性患者中有35例（26%）ICT检测呈假阳性反应，但其中仅4例OptiMAL检测呈假阳性反应。35份假阳性标本中有33份在吸收类风湿因子活性后用ICT检测试剂盒重复检测时变为阴性。我们的研究表明，PfHRP-2为基础的ICT检测给出了一个假阳性反应，在26%的患者有类风湿因子，但疟疾的显微镜检查呈阴性。
Recently introduced rapid nonmicroscopic immunocapture assays for the diagnosis of malaria infection are being evaluated for their sensitivity and specificity in various epidemiological settings. A Plasmodium falciparum histidine-rich protein 2 (PfHRP-2)-based assay (ICT) and a Plasmodium-specific lactate dehydrogenase test (OptiMAL) were evaluated for their specificities in different groups of patients who tested negative for malaria infection by microscopy. The patients were selected from different disease groups: rheumatoid arthritis, hepatitis C, toxoplasmosis, schistosomiasis, and hydatid disease. One hundred thirty-three of the 225 patients were positive for rheumatoid factor. Thirty-five of the 133 (26%) rheumatoid factor-positive patients gave a false-positive reaction with the ICT assay, but only 4 of these gave false-positive reactions with the OptiMAL test. Thirty-three of the 35 false-positive specimens became negative when repeat tested with the ICT assay after absorbing out the rheumatoid factor activity. Our study shows that the PfHRP-2-based ICT assay gave a false-positive reaction in 26% of the patients who had rheumatoid factors, but were negative for malaria by microscopy.