Assessment of regional bone tissue perfusion in rats using fluorescent microspheres.
Assessment of regional bone tissue perfusion in rats using fluorescent microspheres.
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DOI：
10.1016/j.bonr.2017.04.004
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发表时间：
2017-06
期刊：
影响因子：
2.5
通讯作者：
Allen MR
中科院分区：
文献类型：
--
作者：
Aref MW;Akans E;Allen MR
骨血流的干扰已被证明对骨特性具有有害影响，但仍有许多关于健康和疾病中骨骼灌注的未回答的问题，部分原因是测量方法的复杂性。本研究的目的是通过采用小鼠特异性荧光微球方案，在大鼠中使用荧光微球来评估局部骨灌注。10只15周龄的Sprague道利大鼠通过心脏注射（n = 5）或通过尾静脉注射（n = 5）注射荧光微球。采集股骨和胫骨并进行处理以确定组织荧光密度（TFD），该密度与组织毛细血管中捕获的球体数量成正比。在心脏注射动物中评估时，右侧和左侧全股骨TFD（分别为2.77 ± 0.38和2.70 ± 0.24）以及右侧和左侧胫骨TFD（分别为1.11 ± 0.26和1.08 ± 0.34）显示血流双侧对称。将骨灌注沿骨长度分为沿着三段，显示股骨远端和胫骨近端在其各自的骨内接受最大量的灌注。尾静脉注射导致5只动物中4只的胫骨中TFD水平不可接受地低。总之，本报告证明了心脏注射荧光微球评估大鼠骨组织灌注的可行性。
Disturbances in bone blood flow have been shown to have deleterious effects on bone properties yet there remain many unanswered questions about skeletal perfusion in health and disease, partially due to the complexity of measurement methodologies. The goal of this study was use fluorescent microspheres in rats to assess regional bone perfusion by adapting mouse-specific fluorescent microsphere protocol. Ten fifteen-week old Sprague Dawley rats were injected with fluorescent microspheres either via cardiac injection (n = 5) or via tail vein injection (n = 5). Femora and tibiae were harvested and processed to determine tissue fluorescence density (TFD) which is proportional to the number of spheres trapped in the tissue capillaries. Right and left total femoral TFD (2.77 ± 0.38 and 2.70 ± 0.24, respectively) and right and left tibial TFD (1.11 ± 0.26 and 1.08 ± 0.34, respectively) displayed bilateral symmetry in flow when assessed in cardiac injected animals. Partitioning of the bone perfusion into three segments along the length of the bone showed the distal femur and proximal tibia received the greatest amount of perfusion within their respective bones. Tail vein injection resulted in unacceptably low TFD levels in the tibia from 4 of the 5 animals. In conclusion this report demonstrates the viability of cardiac injection of fluorescent microspheres to assess bone tissue perfusion in rats.