Natural Variation in the Splice Site Strength of a Clock Gene and Species-Specific Thermal Adaptation
Natural Variation in the Splice Site Strength of a Clock Gene and Species-Specific Thermal Adaptation
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DOI：
10.1016/j.neuron.2008.10.048
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发表时间：
2008-12-26
期刊：
影响因子：
16.2
通讯作者：
Ederyl, Isaac
中科院分区：
文献类型：
--
作者：
Low, Kwang Huei;Lim, Cecilia;Ederyl, Isaac
我们发现，多个次优剪接位点是D.这一机制可能会减少温带气候下较长夏日里高温的有害影响。In D. yakuba和D. santomea，其具有非洲赤道地区特有的更古老的分布，其中白天长度和温度全年几乎没有波动，其每个3 '末端内含子的剪接效率没有表现出热校准，这与温度对这些物种中活性的每日分布的影响很小一致。我们提出dmpi8上的弱剪接位点是促进广泛定殖的D. melanogaster（可能还有D. simulans）对温带气候的适应性，剪接信号水平上的自然选择在生命形式的热适应中起着重要作用。
We show that multiple suboptimal splice sites underlie the thermal-sensitive splicing of the period (per) 3'-terminal intron (dmpi8) from D. melanogaster, enabling this species to prolong its midday "siesta," a mechanism that likely diminishes the deleterious effects of heat during the longer summer days in temperate climates. In D. yakuba and D. santomea, which have a more ancestral distribution indigenous to Afro-equatorial regions wherein day length and temperature exhibit little fluctuation throughout the year, the splicing efficiencies of their per 3'-terminal introns do not exhibit thermal calibration, consistent with the little effect of temperature on the daily distribution of activity in these species. We propose that the weak splice sites on dmpi8 underlie a mechanism that facilitated the acclimation of the widely colonized D. melanogaster (and possibly D. simulans) to temperate climates and that natural selection operating at the level of splicing signals plays an important role in the thermal adaptation of life forms.