A PARTITION ASSAY FOR THE SIMULTANEOUS DETERMINATION OF INSECT JUVENILE-HORMONE ESTERASE AND EPOXIDE HYDROLASE ACTIVITY
A PARTITION ASSAY FOR THE SIMULTANEOUS DETERMINATION OF INSECT JUVENILE-HORMONE ESTERASE AND EPOXIDE HYDROLASE ACTIVITY
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DOI：
10.1016/0003-2697(88)90257-6
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发表时间：
1988-02-15
影响因子：
2.9
通讯作者：
ROE, RM
中科院分区：
文献类型：
--
作者：
SHARE, MR;ROE, RM
建立了一种测定昆虫保幼激素（JH）酯酶和JH环氧化物水解酶活性的生物样品中JH I和III代谢的分配法。该测定利用市售的放射性链3 H标记的JH作为底物和选择性JH酯酶抑制剂3-辛硫基-1，1，1-三氟-2-丙酮。JH底物与抑制和非抑制样品孵育后，JH分配到异辛烷相中，代谢产物JH酸、JH二醇和JH二醇-酸进入甲醇水溶液中。该测定提供了一种时间和成本效益的替代目前可用的薄层色谱法用于测量JH酯酶和环氧化物水解酶活性。
A partition assay was developed to measure insect juvenile hormone (JH) I and III metabolism in biological samples containing both JH esterase and JH epoxide hydrolase activity. The assay utilizes commrrcially available radiochain 3H-labeled JH as substrate and the selective JH esterase inhibitor 3-octylthio-1,1,1-trifluoro-2-propanone. JH partitions into an isooctane phase and the metabolites JH acid, JH diol, and JH diol-acid into aqueous methanol after incubation of JH substrate with inhibited and unhibited sample. The assay provides a time- and cost-efficient alternative to the currently available thin-layer chromatography method for the measurement of JH esterase and epoxide hydrolase activity.