Molecular imaging of hydrogen peroxide produced for cell signaling
Molecular imaging of hydrogen peroxide produced for cell signaling
复制标题
DOI：
10.1038/nchembio871
复制
发表时间：
2007-05-01
影响因子：
14.8
通讯作者：
Chang, Christopher J.
中科院分区：
文献类型：
--
作者：
Miller, Evan W.;Tulyanthan, Orapim;Chang, Christopher J.
过氧化氢（H2O2）正在成为细胞信号转导中新认识的信使（1，2）。然而，在阐明其在复杂的生物环境中的不同作用的一个重大挑战是缺乏方法来探测这种活性氧代谢物在生命系统中的分子特异性。在这里，我们报告的合成和应用的过氧绿色1（PG 1）和过氧深红1（PC 1），两个新的荧光探针，显示高选择性过氧化氢，并能够可视化内源性过氧化氢在活细胞中产生的生长因子刺激，包括脑细胞信号产生的过氧化物的第一个直接成像。活性氧选择性、对H2O2信号传导水平的敏感性和活细胞相容性的组合特征预示着PG 1、PC 1和相关合成试剂用于利用分子成像探索H2O2在活系统中的生理作用的许多新机会。
Hydrogen peroxide ( H2O2) is emerging as a newly recognized messenger in cellular signal transduction(1,2). However, a substantial challenge in elucidating its diverse roles in complex biological environments is the lack of methods for probing this reactive oxygen metabolite in living systems with molecular specificity. Here we report the synthesis and application of Peroxy Green 1 ( PG1) and Peroxy Crimson 1 ( PC1), two new fluorescent probes that show high selectivity for H2O2 and are capable of visualizing endogenous H2O2 produced in living cells by growth factor stimulation, including the first direct imaging of peroxide produced for brain cell signaling. The combined features of reactive oxygen species selectivity, sensitivity to signaling levels of H2O2, and live- cell compatibility presage many new opportunities for PG1, PC1 and related synthetic reagents for exploring the physiological roles of H2O2 in living systems with molecular imaging.