Lactobacillus sicerae sp. nov., a lactic acid bacterium isolated from Spanish natural cider
Lactobacillus sicerae sp. nov., a lactic acid bacterium isolated from Spanish natural cider
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DOI：
10.1099/ijs.0.059980-0
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发表时间：
2014-09-01
影响因子：
2.8
通讯作者：
Teresa Duenas, M.
中科院分区：
文献类型：
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作者：
Isabel Puertas, Ana;Arahal, David R.;Teresa Duenas, M.
菌株CUPV 261（T）和CUPV 262于2007年从西班牙巴斯克地区的粘稠天然苹果酒中分离。细胞为革兰氏染色阳性、无孢子形成、活动杆状、兼性厌氧菌和过氧化氢酶阴性。该菌株是专性同型发酵的（最终产物DL-乳酸盐），并从蔗糖中产生胞外多糖。基于16 S rRNA基因序列的系统发育分析显示，这两个分离物的最高相似性对应于葡萄酒乳杆菌的模式菌株（99.1%），其次是温州乳杆菌（96.4%）和酒乳杆菌（96.2%），和所有其他已建立的物种，16 S rRNA基因序列的相似性低于96%。利用RAPD指纹图谱对CUPV 261（T）和CUPV 262进行了种属划分。此外，对菌株CUPV 261（T）进行随机部分基因组焦磷酸测序方法，以将其与酿酒乳杆菌DSM 20605（T）的基因组序列进行比较，并计算它们之间的平均核苷酸同一性（ANI）指数。结果表明菌株CUPV 261（T）和CUPV 262代表乳杆菌属的一个新种，并建议将其命名为乳酸杆菌干燥亚种。模式菌株为CUPV 261（T）（=CECT 8227（T）=KCTC 21012（T））。
Strains CUPV261(T) and CUPV262 were isolated from ropy natural ciders of the Basque Country, Spain, in 2007. Cells are Gram-stain positive, non-spore-forming, motile rods, facultative anaerobes and catalase-negative. The strains are obligately homofermentative (final product DL-lactate) and produce exopolysaccharides from sucrose. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the highest similarity to both isolates corresponded to the type strain of Lactobacillus vini (99.1%), followed by Lactobacillus satsumensis (96.4%), and Lactobacillus oeni (96.2%), and for all other established species, 16S rRNA gene sequence similarities were below 96%. The species delineation of strains CUPV261(T) and CUPV262 was evaluated through RAPD fingerprinting. In addition, a random partial genome pyrosequencing approach was performed on strain CUPV261(T) in order to compare it with the genome sequence of Lactobacillus vini DSM 20605(T) and calculate indexes of average nucleotide identity (ANI) between them. Results permit the conclusion that strains CUPV261(T) and CUPV262 represent a novel species of the genus Lactobacillus, for which the name Lactobacillus sicerae sp. nov. is proposed. The type strain is CUPV261(T) (=CECT 8227(T)=KCTC 21012(T)).