Effect of urate on nitric oxide microcirculatory response in the rat tail to body heating.
Effect of urate on nitric oxide microcirculatory response in the rat tail to body heating.
复制标题
尿酸盐对大鼠尾部对身体加热一氧化氮微循环反应的影响。
DOI：
10.1038/sj/mn/7800125
复制
发表时间：
2002
期刊：
影响因子：
--
通讯作者：
Bruckner,G
中科院分区：
文献类型：
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作者：
WidanskiI,B;Richardson,D;Bruckner,G
本研究的目的是确定是否一氧化氮（NO）介导的皮肤血管舒张取决于尿酸concentration.MethodsCapillary血细胞速度（BCV）在麻醉，热应激的Sprague-Dawley大鼠的尾部毛细血管的videomicroscopy方法进行了测量，和股平均动脉压（MAP）记录在Grass测谎仪。通过使用这些化合物的特异性抑制剂，例如，别嘌呤醇（All）或All或N-硝基-L-精氨酸（NNA），分别测定尿酸盐或尿酸盐和NO产生对上述参数的影响。尿酸盐浓度测定酶，比色法，和总亚硝酸盐的水平进行了测定fluorometric.ResultsDuring热stress. ResultsAll或所有和NNA显着降低血浆尿酸盐或尿酸盐和总亚硝酸盐浓度，分别，和血管传导的反应（估计作为一个比率的BCV MAP）。尿酸管理后，所有和NNA预处理，恢复血浆尿酸盐和总亚硝酸盐浓度和增加的BCV/MAP.ConclusionsUrate在生理浓度有助于维持大鼠尾部皮肤毛细血管的血管传导性在身体加热。尿酸盐对血管传导性的影响可能是由NO.Microcirculation（2002）9，125 -131介导的。DOI：10.1038/sj/mn/7800125
ObjectiveThe objective of this study was to determine if nitric oxide (NO)-mediated cutaneous vasodilation depends on urate concentrations.MethodsCapillary blood cell velocities (BCV) in the tail capillary vessels of anesthetized, heat-stressed Sprague-Dawley rats were measured by the videomicroscopy method, and the femoral mean arterial pressure (MAP) was recorded on a Grass polygraph. The effects of urate or urate and NO production on the above parameters were determined by using specific inhibitors of these compounds, for example, allopurinol (All) or All or N-nitro-L-arginine (NNA), respectively. Urate concentrations were determined by an enzymatic, colorimetric method, and total nitrite levels were measured fluorometrically.ResultsDuring heat stress, All or All and NNA significantly decreased plasma urate or urate and total nitrite concentrations, respectively, and the response of vascular conductance (estimated as a ratio of BCV to MAP). Urate administration, after All and NNA pretreatment, restored plasma urate and total nitrite concentrations and increased BCV/MAP.ConclusionsUrate at physiological concentration helps to maintain vascular conductance of cutaneous capillaries in the rat tail during body heating. The effect of urate on vascular conductance may be mediated by NO.Microcirculation(2002)9,125–131. DOI: 10.1038/sj/mn/7800125