Isolation and identification of bacteria responsible for simultaneous anaerobic ammonium and sulfate removal
Isolation and identification of bacteria responsible for simultaneous anaerobic ammonium and sulfate removal
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厌氧氨氮和硫酸盐同时去除细菌的分离和鉴定
DOI：
10.1007/s11426-010-0053-8
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发表时间：
2010-03-01
影响因子：
9.6
通讯作者：
Zheng Ping
中科院分区：
文献类型：
--
作者：
Cai Jing;Jiang JianXiang;Zheng Ping
硫酸盐依赖型厌氧氨氧化是一种新型的生物反应，在缺氧条件下，铵以硫酸盐作为电子受体被氧化。铵和硫酸盐是全球广泛存在的化学物质，是全球氮和硫循环不可或缺的一部分。对硫酸盐依赖型厌氧氨氧化进行详细探究具有很强的实用性。在这项工作中，从一个处于稳定运行状态的厌氧氨和硫酸盐去除反应器中分离出了一株名为ASR的细菌菌株。通过电子显微镜观察、生理测试以及16S rDNA系统发育序列分析，发现菌株ASR与食苯芽孢杆菌（Bacillus benzoevorans）有关。根据生物碳源利用测试，菌株ASR能够利用多种碳源。其最适pH值和温度分别为8.5和30℃。测试证明菌株ASR能够利用硫酸盐厌氧氧化氨。氨和硫酸盐的最大去除率分别为44.4%和40.0%。本研究为硫酸盐依赖型厌氧氨氧化的确认和发展提供了生物学证据，并为全球氮和硫循环带来了新的见解。
Sulfate-dependent anaerobic ammonium oxidation is a novel biological reaction, in which ammonium is oxidized with sulfate as the electron acceptor under anoxic conditions. Ammonium and sulfate are cosmopolitan chemical species which are an integral part of the global nitrogen and sulfur cycles. A detailed exploration of sulfate-dependent anaerobic ammonium oxidation is quite practical. In this work, a bacterial strain named ASR has been isolated from an anaerobic ammonia and sulfate removing reactor working under steady-state. On the basis of electron microscopy, physiological tests and 16S rDNA phylogenetic sequence analysis, the strain ASR is found to be related toBacillus benzoevorans. According to the biological carbon source utilization test, the strain ASR could use many carbon sources. Its optimum pH value and temperature were 8.5 and 30 °C, respectively. The test proves that the strain ASR is able to use sulfate to oxidize ammonia anaerobically. The maximum ammonia and sulfate removal rates were 44.4% and 40.0%, respectively. The present study provided biological evidence for the confirmation and development of sulfate-dependent anaerobic ammonium oxidation and brought new insights into the global nitrogen and sulfur cycles.